Introduction Toxoplasma gondii is an obligate intracellular parasite that can infect a broad range of warmblooded animals. In humans, it can cause serious disease in immune-compromised individuals or if contracted congenitally. In North America and Europe, strains belonging to the clonal types I, II, and III haplogroups predominate, while in South America a large variety of other “atypical” str...

To evaluate the usefulness of different commercial enzyme linked immunosorbent assays (ELISAs) for the detection of IgM antibodies against Toxoplasma gondii the results of six of these assays for a panel of 81 sera were compared. The following tests were selected: Toxoplasma gondii IgM ELISA (Clark Laboratories), Toxoplasma IgM EIA (Labsystems), Toxo-M EIA (Abbott), Toxonostika M (Organon), Tox...

A direct enzyme-linked immunosorbent assay (ELISA) is described that uses horseradish peroxidase-labeled antigen for detection of immunoglobulin M (IgM) and IgA antibodies to toxoplasma. In this assay, polystyrene microtiter plates were sensitized with anti-human IgM or IgA antibody to separate IgM or IgA from other classes of antibody. The presence of IgM or IgA antibodies to toxoplasma (Tox-I...

BACKGROUND Toxoplasmosis is typically diagnosed by serologic testing. External quality assessment (EQA) of clinical laboratories could ensure the accuracy and reliability of serological tests. We assessed the quality of toxoplasma serological assays in Chinese clinical laboratories by an EQA performed between 2004 and 2013 by the National Center for Clinical Laboratories. METHODOLOGY AND FIND...

BACKGROUND Toxoplasmosis is a parasitic disease caused by the protozoan Toxoplasma gondii. This parasite infects most of warm-blooded animals, including birds. Turkeys are one of these animals which might be infected by this parasite. Little is known about the prevalence of T. gondii in turkeys in Iran. OBJECTIVES The current study aimed to evaluate the rate of Toxoplasma infection in turkeys...

From 1st Jan2009 to31th of Dec 2009 a total 1406 women sera with abortions and outcomes congenital abnormalities in Kirkuk city were screened for infections like Chlamydia trachomatis, Toxoplasma gondii cytomegalovirus (CMV).These agents determined using commercial Elisa diagnostic kits.
 Results the study showed that CMV infection was more prevalent 39.74 % followed by Toxoplasmosis 16.37...

Toxoplasma gondii (T. gondii) is a protozoan parasite, infecting most of the warm-blooded animals, including humans who are the intermediate host. Members of cat family Felidae are the definitive hosts. T. gondii infection is acquired by humans by accidental ingestion of oocysts in water, food or soil contaminated with cat feces, or by eating raw or undercooked meat containing cysts. Studies co...

Toxoplasma gondii is an obligate intracellular parasite that infects a wide variety of vertebrate cells including macrophages. We have used a combination of video microscopy and fluorescence localization to examine the entry of Toxoplasma into macrophages and nonphagocytic host cells. Toxoplasma actively invaded host cells without inducing host cell membrane ruffling, actin microfilament reorga...

Toxoplasma gondii is a protozoan parasite that persists as a chronic infection. Toxoplasma evades immunity by forming tissue cysts, which reactivate to cause life-threatening disease during immune suppression. There is an urgent need to identify drugs capable of targeting these latent tissue cysts, which tend to form in the brain. We previously showed that translational control is critical duri...

Present on the plasma membrane of most metazoans, caveolae are specialized microdomains implicated in several endocytic and trafficking mechanisms. Caveolins and the more recently discovered cavins are the major protein components of caveolae. Previous studies reported that caveolar invaginations can be induced de novo on the surface of caveolae-negative mammalian cells upon heterologous expres...