RNA sequencing approaches to transcriptome analysis require a large amount of input total RNA to yield sufficient mRNA using either poly-A selection or depletion of rRNA. This feature makes it difficult to miniaturize transcriptome analysis for greater efficiency. To address this challenge, we devised and validated a simple procedure for the preparation of whole-transcriptome cDNA libraries fro...

trichophyton rubrum is an anthropophilic fungus causing up to 90% of chronic cases of dermatophytosis. several properties of this fungus have been investigated so far. however, a few studies were carried out in the field of molecular biology of this fungus. in the present study, we tried to identify the subunit g of its vacuolar atpase (v-atpase). pairs of 21 nt primers were designed from highl...

RNA plays a central role in the determination of the phenotype of the cell. The molecular mechanisms involved in primary RNA synthesis and subsequent post-processing are not completely understood, but there is increasing evidence that they are more tightly coupled than previously expected. The analyses by a number of groups of recently published genome wide maps of chromatin structure have furt...

Background: Varicocele (VCL) impacts the testicular tissue partly by down-regulating the antioxidant status accomplished with reducing the intra-testicular endocrine potential. It has been reported that the HSP70 families play a critical role in spermiogenesis. Therefore, current study was aimed to evaluate the protective effect of exogenous testosterone and vitamin E administration on VCL-indu...

MicroRNAs (miRNAs) are key biological regulators and promising disease markers whose detection technologies hold great potentials in advancing fundamental research and medical diagnostics. Currently, miRNAs in biological samples have to be labeled before being applied to most high-throughput assays. Although effective, these labeling-based approaches are usually labor-intensive, time-consuming ...

We have developed a procedure for isolation of microRNA and genomic DNA in addition to total RNA from whole blood stabilized in PAXgene Blood RNA tubes. The procedure is based on automatic extraction on a BioRobot MDx and includes isolation of DNA from a fraction of the stabilized blood and recovery of small RNA species that are otherwise lost. The procedure presented here is suitable for large...

The RNA 6000 LabChip® kit can be used with the Agilent Technologies 2100 bioanalyzer to determine the integrity and measure the concentration of total RNA samples obtained from various mouse tissues and from E. coli. For each sample the data analysis software automatically calculated the ratio of ribosomal RNA peaks and reported the total RNA concentration in ng/μl. The resolution of the RNA se...