A yolk protein enzyme-linked immunosorbent assay (YP-ELISA) was developed for the predator Orius insidiosus (Say). The YP-ELISA is intended to assess reproductive response to dietary and other rearing conditions, and to assist in quality control and diet development for mass rearing. Hybridomas and monoclonal antibodies were produced against homogenates of eggs dissected from females. Hybridoma...

Rheumatoid arthritis (RA) is one of the most common autoimmune diseases, affecting 0.5% to 1% population. It could ultimately result in joint destruction, functional decline, work disability, and enhanced mortality. Cyclic citrullinated peptide antibodies (CCP Abs) are useful biomarkers for early detection diagnosis RA. In this study, we used plant viral-based expression vectors that produce ra...

The recent emergence of the human infection confirmed to be caused by severe fever with thrombocytopenia syndrome virus (SFTSV) in China is of global concern. Safe diagnostic immunoreagents for determination of human and animal seroprevalence in epidemiological investigations are urgently needed. This paper describes the cloning and expression of the nucleocapsid (N) protein of SFTSV. An N-prot...

background: phthalates are a group of multifunctional chemicals. diethyl phthalate (dep) is one of the most frequently used phthalates in solvents and fixatives for numerous industrial products. method: the present experiment was designed to assess oxidative stress, if any, caused by diethyl phthalate. for this the homogenates of liver and kidney were treated with different concentrations ( 10-...

Background: Dental caries is one of the most common oral diseases in pre-school children. Several factors can affect caries process. Aim of this study was comparison of some of the chemical properties of saliva such as total antioxidant capacity, total protein, pH, nitric oxide level in caries free (CF), and caries active (CA) children.<...

Objective(s): Production of a recombinant and immunogenic antigen using dengue virus type-3 envelope protein is a key point in dengue vaccine development and diagnostic researches. The goals of this study were providing a recombinant protein from dengue virus type-3 envelope protein and evaluation of its immunogenicity in mice. Materials and Methods: Multiple amino acid sequences of different i...

This study was carried out to determine serum haptoglobin levels of pregnant cows with complications during pregnancy. A total 28 animals were included in the study, 14 healthy birth and complicated pregnancies, whose processes followed same farm. Blood samples taken from vena jugularis into yellow capped biochemistry tubes at 6th month their Serum albumin, globulin protein measurements made an...

Abbreviations: BSA: bovine serum albumin CHO: Chinese hamster ovary CL: total plasma clearance Cmax: maximum plasma concentration ELISA: enzyme-linked immunosorbent assay FCS: fetal calf serum ip: intraperitoneal iv: intravenous PBS: phosphate-buffered saline rhGM-CSF: recombinant human granulocyte-macrophage colony-stimulating factor t1/2 α: distribution half-life t1/2 : β terminal half-life T...

Affinity purified anticardiolipin antibodies (ACLA) raised in rabbits showed cross-reactivities with various negatively charged phospholipids as shown by both the solid phase enzyme-linked immunosorbent assay (ELISA) and inhibition studies. In ELISA, ACLA showed strong cross-reactivity to both sphingomyelin (SM) and phosphatidylethanolamine (PE), but the inhibition studies showed that ACLA fail...