BACKGROUND Failed fertilization occurs in 2-3% of ICSI cycles and is mainly due to lack of oocyte activation. Heterologous ICSI of patient's sperm in mouse oocytes allows discrimination between sperm- and oocyte-related aetiologies of activation failure. Assisted oocyte activation (AOA) by Ca-ionophore treatment can initiate fertilization in subsequent therapeutic ICSI. We report on diagnosis a...

Sperm-head elongation and acrosome formation, which take place during the last stages of spermatogenesis, are essential to produce competent spermatozoa that are able to cross the oocyte zona pellucida and to achieve fertilization. During acrosome biogenesis, acrosome attachment and spreading over the nucleus are still poorly understood and to date no proteins have been described to link the ac...

Fluorescence in situ hybridization analysis, performed with chromosome specific DNA probes labeled with fluorochromes, is a simple and reliable tool for the indirect study of aneuploidies in interphase cells such as spermatozoa. It is known that infertile male patients with poor sperm quality, due to different causes, produce cytogenetically abnormal spermatozoa despite a normal constitutional ...

LINC complexes are evolutionarily conserved nuclear envelope bridges, physically connecting the nucleus to the peripheral cytoskeleton. They are pivotal for dynamic cellular and developmental processes, like nuclear migration, anchoring and positioning, meiotic chromosome movements and maintenance of cell polarity and nuclear shape. Active nuclear reshaping is a hallmark of mammalian sperm deve...

background: in the recent years, eutrophication has become a serious challenge for many of the water ecosystems. it has affected the environmental and economical aspects of the anzali wetland, which is known as one of the significant ecosystem. due to the increase of the nutrient loadings from the industrial, agricultural and human activities, the wetland’s health is faced many serious problems...

present transplant study was carried out across lucknow, the capital of utter pardesh using two medicinally important plants viz., ?catharanthus roseus l.? and ?ocimum sanctum l.? to assess whether certain ecophysiological responses (change in total flavonoids and total phenolics) in these two plants may serve as the biomarkers of auto exhaust pollution. samples were taken from five different s...