background: because of the strong immunologic responses of surface protein tasp in theileria annu­lata infected host, we tried to characterize this protein in a t. annulata isolate from iran. methods: the rna prepared from t. annulata infected cells was used to produce smart-ds-cdna. the double strand cdna was then amplified with primers derived from tasp mrna se­quences. the pcr product was cl...

We have cloned and sequenced a cDNA for a histone H2A from parsley (Petroselinum crispum). A library of parsley cDNA in lambda gtll was probed with a cloned fragment originally generated by polymerase chain reaction (PCR). The DNA amplified by PCR was a cDNA preparation made using the downstream PCR oligo primer with a total RNA preparation from parsley cell culture. The PCR upstream primer (a ...

cDNA clones comprising the entire coding region of two out of the seven heterogeneous subunits of hemocyanin from the tarantula, Eurypelma californicum, were isolated from four cDNA libraries constructed from total RNA from the heart tissue of single spiders. Hybridization was first carried out using a tarantula hemocyanin subunit e partial cDNA, and several positive clones were isolated, inclu...

a cDNA library transfer system based on retroviral vectors has been developed for expression cloning in mammalian cells. The use of retroviral vectors results in stable cDNA transfer efficiencies which are at least 100-fold higher than those achieved by transfection and therefore enables the transfer and functional screening of very large libraries. In our initial application of retroviral tran...

A comparative study of the gene expression profile in different developmental stages of Schistosoma mansoni has been initiated based on the expressed sequence tag (EST) approach. A total of 1401 ESTs were generated from seven different cDNA libraries constructed from four distinct stages of the parasite life cycle. The libraries were first evaluated for their quality for a large-scale cDNA sequ...

In this study, a cDNA library was constructed from the total RNA of sweet potato leaves. A total of 789 copies of the cDNA were cloned in Escherichia coli by employing the pGEM-T Easy vector. Sequencing was carried out by Solgent Co. (Korea). As many as 579 expressed sequence tag-simple sequence repeat (EST-SSR) markers were designed (73.38%) from the known cDNA nucleotide base sequences. The l...

A strain of tobacco mosaic virus (TMV) named TMV Korean common strain has been isolated in Suwon (South Korea) from Nicotiana tabacum NC82. The full-length double stranded cDNA of viral RNA has been synthesized and cloned. Three oligonucleotide primers (5' ACTTAAGAATATTAGAAT 3'; 5' GTAGTACTTGAACGAACA 3'; 5' TGGGCCCCTACCGGG 3'), which had been found to be complementary to each of TMV RNA residue...

The sources of variance and errors in transcriptomics based on the electrophoretic separation of amplified cDNA fragments were investigated using cDNA-amplified fragment length polymorphism (AFLP). Transcriptome profiles of the plant-pathogenic fungus Verticillium longisporum were generated by a standard cDNA-AFLP protocol followed by electrophoretic separation of amplified DNA fragments in fla...

There is increasing interest in interfacing dissociated neuronal cultures with planar multielectrode arrays (MEAs) for the study of the dynamics of neuronal networks. Here we report on the successful use of three-dimensional tip electrode arrays (3D MEAs), originally developed for use with brain slices, for recording and stimulation of cultured neurons. We observed that many neurons grew direct...

BACKGROUND Construction of high quality cDNA libraries from the usually low amounts of eukaryotic mRNA extracted from environmental samples is essential in functional metatranscriptomics for the selection of functional, full-length genes encoding proteins of interest. Many of the inserts in libraries constructed by standard methods are represented by truncated cDNAs due to premature stoppage of...