Ethylene glycol (EG) consumption is commonly employed as an experimental regimen to induce hyperoxaluria in animal models of calcium oxalate nephrolithiasis. This approach has, however, been criticized because EG overdose induces metabolic acidosis in humans. We tested the hypothesis that EG consumption (0.75% in drinking water for 4 wk) induces metabolic acidosis by comparing arterial blood ga...

This in vitro study sought to evaluate nine different sport and energy drinks (B Star Energy drink, London Best Energy Drink, Hippo Energy drink, Lucozade Boost Energy fast, Hype Energy, Vita 500 Energy drink, Lucozade Sport, Power horse Energy drink and Red Bull Energy drink) in terms of initial pH and titratable acidity. Initial pH was measured in triplicate for the nine beverages. The titrat...

Titratable systems are common tools in metabolic engineering to tune the levels of enzymes and cellular components as part of pathway optimization. For nonmodel microorganisms with limited genetic tools, inducible sugar utilization pathways offer built-in titratable systems. However, these pathways can exhibit undesirable single-cell behaviors that hamper the uniform and tunable control of gene...

Molecular dynamics simulations of triclinic hen egg white lysozyme in aqueous solution were performed to calculate the intrinsic pKas of 14 ionizable residues. An all-atom model was used for both solvent and solute, and a single 180 ps simulation in conjunction with a Gaussian fluctuation analysis method was used. An advantage of the Gaussian fluctuation method is that it only requires a single...

The rational use of nutrients is of fundamental importance for obtaining high productivity of high nutritional quality. This work was developed with the aim of evaluating the quality of pepper fruit grown under different fertigation managements and different levels of nitrogen and potassium. The experimental design was of randomised blocks in a 3 x 6 factorial scheme with four replications. The...

The postharvest treatments included control, low temperatures (14 and 18°C) and plastic bags with or without perforations. Changes in different physico-chemical characteristics of fruits were studied during storage. The two factor experiment was laid out in completely randomized design with three replications of 10 fruits. Among the physico-chemical parameters, colour, firmness, total weight lo...

In constant pH molecular dynamics simulations, the protonation states of titratable sites can respond to changes of the pH and of their electrostatic environment. Consequently, the number of protons bound to the biomolecule, and therefore the overall charge of the system, fluctuates during the simulation. To avoid artifacts associated with a non-neutral simulation system, we introduce an approa...

BACKGROUND Two important quality attributes of processing tomatoes are pH and titratable acidity. These and other quality attributes can be affected by tomato fruit maturity and over-maturity. We have determined the magnitude of these maturity effects in four processing tomato cultivars commonly grown in California. RESULTS Allowing tomatoes to remain on the vine for up to 4 weeks after ripen...

INTRODUCTION One of the most frequent parents' concerns is that oral antibiotic formulations induce dental damage in their children's. This study aimed to assess the cariogenic and erosive potentials of 29 pediatric antibiotics. MATERIALS AND METHODS Replicates of each antibiotic were analyzed for the concentration of sugars (sucrose, glucose and fructose) and sorbitol by high performance liq...

In connection with global warming, study to find new technological solutions that allow obtain wines characterized by a lower ethanol content and balanced in the acids sugars is relevant.One of use Lachancea thermotolerans yeast, which can metabolize some hexoses into lactic acid. present work comparative studies chemical composition from grape cultivar Cabernet Sauvignon Crimean autochthonous ...