We describe an ultrasensitive, enzymatically amplified time-resolved fluorescence immunoassay of thyrotropin (thyroid-stimulating hormone) in serum with use of a terbium chelate as the detectable moiety. In this assay, thyrotropin is first simultaneously reacted with a solid-phase (microtiter well) monoclonal antibody and a soluble biotinylated monoclonal detection antibody. After washing, a st...

Within the quality control scheme of the Deutsche Gesellschaft für Klinische Chemie, seven quality control surveys of thyrotropin (TSH) determinations in blood dried on filter paper have been carried out since 1980. Ninety-six screening laboratories from 12 European countries took part in these surveys. In a single survey each participant usually analysed four different samples; each of these c...

BACKGROUND Thyroid dysfunction increases with age. Less is known about the prevalence of thyroid disease in older black adults and whether an association between thyroid function and serum cholesterol level exists, as in older white adults. METHODS A cross-sectional study of 2799 well functioning white and black participants, aged 70 to 79 years, were recruited for a population-based study. P...

entiated tumors. The first-generation transplantable tumors are usually differentiated, functional, and thyrotropin dependent. The tumors of later generations are often poorly differentiated, nonfunctional, and thyrotropin independent. Williams and Doniach (15) observed growth of thyroid autotransplants in hypophysectomized rats and in rats with thyrotropin secretion suppressed by administratio...

Endogenous thyrotropin-releasing hormone (TRH) reserve and pituitary thyrotropin (TSH) reserve were assessed in four normal subjects, three patients post-cryohypophysectomy, one patient with a hypothalamic lesion secondary to trauma, and four patients with Sheehan's syndrome. TSH reserve was determined by the immunoassayable TSH response to 500 mug TRH given i.v. (TRH stimulation test). TRH res...

In two patients with congenital isolated thyrotropin (TSH) deficiency, serum TSH determined by a sensitive immunoradiometric assay (IRMA) was consistently undetectable. The basal levels of serum free TSH-alpha subunit (TSH-alpha) determined by a specific radioimmunoassay (RIA) were elevated in the hypothyroid state, and decreased to the undectable level during displacement therapy with thyroid ...

Indirect single- and double-staining immunofluorescence techniques were used to study the serotoninergic, peptidergic and GABAergic innervation of the ventrolateral (Onuf's nucleus) and dorsolateral (innervating intrinsic foot sole muscles) nuclei, located in the S1/S2 segments of the cat spinal cord. The relative density of 5-hydroxytryptamine-, thyrotropin-releasing hormone-, substance P- and...

Introduction: Graves Disease (GD) is the most common cause of primary hyperthyroidism. It an autoimmune disorder characterized by presence thyrotropin related antibodies that stimulate thyroidal cells, resulting in overproduction thyroid hormones. has been reported cytokines might be involved GD pathogenesis. So, aim present study was to investigate possible associations between IL-37 Single Nu...