Cytosine arabinoside and Methotrexate alter in different ways the metabolic patterns of leukocytes from patients with acute granulocytic leukemia. The variations in the incorpora tion into DNA of deoxyuridine and thymidine during the first twenty-four hours after cytosine arabinoside administration were correlated with one another but not with the variation in activity of thymidylate synthetase...

A convenient and efficient method for deprotection of N-(trimethyl)silylethoxymethyl (SEM) groups from thymidine dinucleoside and dinucleotide has been achieved. The SEM groups were easily removed in excellent yields from protected nucleosides, dinucleosides, and dinucleotides.

Infection with high-risk human papillomavirus (HPV) types is linked to the onset of several cancers. The mechanism HPV infection, however, has not yet been fully elucidated. Here, using newly developed infectious pseudovirion (HPV PsV) and a genome-wide clustered regularly interspaced short palindromic repeat (CRISPR) screening system, we established an experimental system searched for genes in...

Positron emission tomography can be used to image tumor proliferation when combined with appropriate labeled tracers, such as the thymidine analog [(18)F]-3'-deoxy-3'-fluorothymidine. Although thymidine kinase 1 is the principal mechanism of cell trapping, other variables, such as the cellular level of native thymidine, may need to be considered.

We studied the expression of 11 cell cycle-dependent genes in senescent WI-38 fibroblasts and compared the results to those obtained in WI-38 cells from early passages (young cells). Every gene we examined is expressed in the senescent cells at levels similar to those in the young cells, including two genes maximally expressed at the G1/S phase boundary--genes for thymidine kinase and histone H...

YBRIDS between mammalian cells with known biochemical differences permit the study of the interactions of the two parental genomes in a single cell (EPHRUSSI 1965; HARRIS 1966). Resistance to 5-bromo-2’-deoxyuridine (BUdR) in mammalian cell lines was accompanied by an inability of the resistant cell to incorporate BUdR into DNA, presumably as a result o€ the simultaneous loss of thymidine kinas...

Growth inhibition by methotrexate (MTX) of a cultured mouse mammary carcinoma FM3A line and its mutants deficient in thymidine kinase and thymidylate synthetase was studied un der a variety of conditions. In medium containing 10 ¡IMthy midine, the thymidylate synthetase-deficient mutants were slightly more resistant to MTX than the wild-type line and the thymidine kinase-deficient mutant. The ...

The biosynthetic enzymes DNA polymerase, thymidine kinase (TdR kinase) and thymidine monophosphate kinase (dTMP kinase) show characteristic patterns of activity associated with cell division in synchronously dividing tissue cultures of the Jerusalem artichoke. In each case the first major increase in enzyme activity is coincident with and dependent upon DNA replication. In addition the increase...

We quantify cytosolic and mitochondrial deoxyribonucleoside triphosphates (dNTPs) from four established cell lines using a recently described method for the separation of cytosolic and mitochondrial (mt) dNTPs from as little as 10 million cells in culture (Pontarin, G., Gallinaro, L., Ferraro, P., Reichard, P., and Bianchi, V. (2003) Proc. Natl. Acad. Sci. U. S. A. 100, 12159-12164). In cycling...