Currently, most reusable aptasensors rely on the reassembly of aptamers to regenerate sensing interface. However, it remains challenging achieve successive aptasensor reusability. Herein, for first time, we demonstrate that a ratiometric strategy endows electrochemical high reusability realize detection aflatoxin B1 (AFB1). The model interface used thionine (THI)-graphene nanocomposite and ferr...