Two previous studies demonstrated elevated levels of 3-ethyladenine in smokers' urine, suggesting that cigarette smoke may contain a DNA ethylating agent. We hypothesized that such an agent would also lead to elevated levels of N-terminal N-ethylvaline in hemoglobin. N-terminal N-alkylated valines in hemoglobin can be measured using a modified Edman degradation, which employs pentafluorophenyl ...

c-di-AMP is an important bacterial second messenger found in Gram-positive and mycobacteria. c-di-AMP regulates myriads of processes in bacteria as well as immune response in higher organisms so interest in small molecules that would attenuate the activity of c-di-AMP metabolism enzymes is high. Herein, we report the first small molecule inhibitor of a c-di-AMP synthase, DisA, using a coralyne-...

Novel imidazo[2,1-b]thiazoles were synthesized in good yield via a mild reaction of thiophenytoin and diethyl oxalatein the presence of 10% mol p-TSA.

The reaction of stoichiometric amounts of dialkyl acetylenedicarboxylates with alkyl isocyanides and 5,5-diaryl thiohydantoins in toluene and catalytic amount of p-TSA afforded imidazo[2,1-b][1,3]thiazines in good overall yields

The fields of natural product total synthesis and combinatorial chemistry have major differences as well as much in common. Unique to combinatorial chemistry is the need to devise rapid and efficient methods for parallel synthesis and purification, while an area of overlap is the targeting of natural product scaffolds for combinatorial libraries. Both these aspects are illustrated with examples...

A cDNA library was synthesized from maize anaerobic root mRNA and screened with cDNA specific to the anaerobically induced Zea mays cytoplasmic aldolase. At least 1% of the cDNA of the library corresponded to maize cytoplasmic aldolase. The sequence of four overlapping cDNA clones encoded a protein of molecular weight 38,611 homologous to aldolase. These cDNAs were polymorphic at three bases an...

•Folate-O-FITC switch CAR-T cells to an activated state in a dose-dependent manner•CAR-T-induced cytotoxicity can be rapidly terminated by the cleavage of folate-O-FITC•CAR-T turned on/off on demand supplementation/cleavage folate-O-FITC•Such switchable regulated manner would not compromise antitumor effects Chimeric antigen receptor (CAR)-T-based therapeutics are breakthrough cancer treatment;...