329 M ANAGEMENT of the allergic infant often includes extensive dietary restriction in addition to avoidance of milk. A milkfree formula may tilus become a major or even sole SOU CC of most essential nutrients for many Illontils. Furthermore, the occasional coexistence of anorexia an(l intercurrent infection in children with allergic ITI 1Il ifestations, and losses of protein tilrOu gil the ski...

STUDY OBJECTIVE Micronutrient deficiencies have become rare in industrialised countries as availability of fresh food, supplementation, and fortification have improved but a less favourable situation may still prevail in many developing countries. Blood thiamin status and determinants were therefore investigated in the Seychelles in view of the high incidence of dilated cardiomyopathy and as th...

Infections with enteric pathogens like enterotoxigenic Escherichia coli (ETEC) is a major health issue worldwide and while diarrhea is the major problem, prolonged, severe, and dual infections with multiple pathogens may also compromise the nutritional status of the infected individuals. There is almost nothing currently known about the effect of ETEC infection on intestinal absorptions of wate...

Optimum conditions for the assay of thiamin were studied using a cyanogen bromide (BrCN) oxidation method. The adopted procedure included neither pre-purification of samples through an ion exchanger nor extraction of the thiochrome into an organic solvent. The 0.25 M BrCN (the concentration before the addition of alkali) and the final NaOH concentration of approx. 1% gave the highest yield of t...

Due to genetic defects or illness some individuals require higher amounts of thiamin than are typically provided by the diet. Lipid-soluble thiamin precursors can achieve high blood levels of thiamin and result in increased concentrations in the central nervous system. High intakes of thiamin have been reported as beneficial in children with autism and attention deficit/hyperactivity disorder. ...

Thiamin and riboflavin are precursors of essential coenzymes-thiamin pyrophosphate (TPP) and flavin mononucleotide (FMN)/flavin adenine dinucleotide (FAD), respectively. In Bacillus spp, genes responsible for thiamin and riboflavin biosynthesis are organized in tightly controllable operons. Here, we demonstrate that the feedback regulation of riboflavin and thiamin genes relies on a novel trans...

Since 1869 when RAULIN reported that Aspergilluts niger excreted some growth-promoting substances into its medium, a number of investigators have studied the phenomenon of auxithal synthesis by fungi. For a comprehensive review of this the reader is referred to FRIES (2) and SCHLENKER (7). The writers (5) have referred briefly to this subject; the present paper reports a more exhaustive study. ...

Vitamin B1 is a watersoluble vitamin. It consists out of a pyrimidineand a thiazol ring, which are synthesized independently and linked afterwards.Vitamin B1 is de novo produced by many plants and microorganisms. Humans and animals have to take it up by nutrition. They are only able to do the phosphorylation reaction of the thiamin molecule. Thiamin is found free and as mono-, diand triphosphat...

Active transport of thiamin (vitamin B(1)) into Escherichia coli occurs through a member of the superfamily of transporters known as ATP-binding cassette (ABC) transporters. Although it was demonstrated that the sulfhydryl-specific modifier N-ethylmaleimide (NEM) inhibited thiamin transport, the exact mechanism of this inhibition is unknown. Therefore, we have carried out a kinetic analysis of ...

The genes encoding thiamine kinase in Escherichia coli (ycfN) and thiamine pyrophosphokinase in Bacillus subtilis (yloS) have been identified. This study completes the identification of the thiamine salvage enzymes in bacteria.