This study is focused on the development of a systematic computational approach which implements Genetic Algorithm (GA) to find the optimal rigorous kinetic models.A general Kinetic model for hydrogenolysis of dibenzothiophene (DBT) based on Langmuir-Hinshelwood type has been obtained from open literature. This model consists of eight continuous parameters(e.g., Arrhenus  and Van't...

a semi-batch crystallization system is used to determine growth kinetics and morphology of barium carbonate crystals, under a constant ph value and constant temperature in the various relative supersaturations. results show that crystal growth rate satisfies a first-order kinetic expression at high relative supersaturation, while at relative supersaturation values lower than 3,  the kinetic exp...

Beta-galactosidase (ß-D-galactoside galactohydrolase, EC 3.2.1.23), has tremendous potential in research and application in various fields like food, bioremediation, biosensor, diagnosis and treatment of disorders. The sources of the betagalactosidase are microorganisms, plants and animals. Microbial beta-galactosidase is of much interest because of their thermostability, thermoacidophilic and ...

Cytosolic sulfotransferases (SULTs) are mammalian enzymes that detoxify a wide variety of chemicals through the addition of a sulfate group. Despite extensive research, the molecular basis for the broad specificity of SULTs is still not understood. Here, structural, protein engineering and kinetic approaches were employed to obtain deep understanding of the molecular basis for the broad specifi...

Rhus laccase was isolated and purified from acetone powder obtained from the exudates of Chinese lacquer trees (Rhus vernicifera) from the Jianshi region, Hubei province of China. There are two blue bands appearing on CM-sephadex C-50 chromatography column, and each band corresponding to Rhus laccase 1 and 2, the former being the major constituent, and each had an average molecular weight of ap...

Distinct organellar forms of the beta-oxidation enzyme enoyl-coenzyme A (CoA) hydratase were partially purified and characterized from 2-day germinated pea (Pisum sativum L.) seedlings. The purification was accomplished by disruption of purified mitochondria or peroxisomes, (NH(4))(2)SO(4) fractionation, and gel permeation chromatography using a column of Sephacryl S-300. The organellar isozyme...

The extracellular glucoamylase from certain strains of Saccharomyces cerevisiae can be purified from culture medium by a simple chromatographic procedure. The native enzyme is heavily glycosylated and has an Mr of about 250,000, but gel filtration indicates the existence of oligomers of larger size. Dissociation yields a form of Mr about 70,000. The glucoamylase is rich in serine and threonine ...

Exploiting carbonic anhydrase (CA), an enzyme that rapidly catalyzes carbon dioxide hydration, is an attractive biomimetic route for carbon sequestration due to its environmental compatibility and potential economic viability. However, the industrial applications of CA are strongly hampered by the unstable nature of enzymes. In this work, we introduced in silico designed, de novo disulfide bond...

It is often necessary to analyze the time response of a tracer. A common way of analyzing the tracer time response is to use a compartment model and estimate the model parameters. The model parameters are generally physiologically meaningful and called “kinetic parameters”. In this paper, we simultaneously estimate both the kinetic parameters at each voxel and the model-based plasma input funct...

In this paper glucoamylase from Aspergillus niger was immobilized by using a modified version of cross-linked enzyme aggregates (CLEA). The co-aggregates were cross-linked with glutaraldehyde; meanwhile dextrin and xanthan gum as protecting agents were added, which provides high affinity with the enzyme molecules. The immobilized glucoamylase was stable over a broad range of pH (3.0–8.0) and te...