So far retinylidene proteins (∼rhodopsin) have not been discovered in thermophilic organisms. In this study we investigated and characterized a microbial rhodopsin derived from the extreme thermophilic bacterium Thermus thermophilus, which lives in a hot spring at around 75 °C. The gene for the retinylidene protein, named thermophilic rhodopsin (TR), was chemically synthesized with codon optimi...

BACKGROUND Consolidated bioprocessing (CBP) of lignocellulosic biomass to ethanol using thermophilic bacteria provides a promising solution for efficient lignocellulose conversion without the need for additional cellulolytic enzymes. Most studies on the thermophilic CBP concentrate on co-cultivation of the thermophilic cellulolytic bacterium Clostridium thermocellum with non-cellulolytic thermo...

The thermophilic, gram-positive bacterium Thermoterrabacterium ferrireducens coupled organotrophic growth to the reduction of sparingly soluble U(VI) phosphate. X-ray powder diffraction and X-ray absorption spectroscopy analysis identified the electron acceptor in a defined medium as U(VI) phosphate [uramphite; (NH4)(UO2)(PO4) . 3H2O], while the U(IV)-containing precipitate formed during bacter...

IP: 54.191.40.80 On: Fri, 11 Aug 2017 04:47:37 Thermoflexus hugenholtzii gen. nov., sp. nov., a thermophilic, microaerophilic, filamentous bacterium representing a novel class in the Chloroflexi, Thermoflexia classis nov., and description of Thermoflexaceae fam. nov. and Thermoflexales ord. nov. Jeremy A. Dodsworth, Jonathan Gevorkian, Fairuz Despujos, Jessica K. Cole,* Senthil K. Murugapiran, ...

The sporeforming, dissimilatory sulfate-reducing bacteria have been reclassified by L. L. Campbell and J. R. Postgate (Bacteriol. Rev. 29:359, 1965) and placed in a new genus: Desuljbtomaculum. The thermophilic sulfate-reducing bacterium, Desulfotomaculum nigrificans (Clostridium nigrificans) was designated as the type species. In a previous study of 30 strains of nonsporulating sulfate-reducin...

Structural studies of the ribosome have benefited greatly from the use of organisms adapted to extreme environments. However, little is known about the mechanisms by which ribosomes or other ribonucleoprotein complexes have adapted to functioning under extreme conditions, and it is unclear to what degree mutant phenotypes of extremophiles will resemble those of their counterparts adapted to mor...

Extensive enzymatic studies have been conducted on cell-free fractions from a thermophilic bacterium designated as National Canners Association strain 2184 and tentativelv identified as Bacillus stearothermophilus Donk (1920), (Militzer et al., 1949, 1950, 1951, 1952; Georgi et al., 1951; h'Iarsh and Militzer, 1952). Preparatory to extending these investigations to other thermophilic bacteria, ...

Oil fields can experience souring, the reduction of sulfate to sulfide by sulfate-reducing microorganisms. At the Terra Nova oil field near Canada's east coast, with a reservoir temperature of 95°C, souring was indicated by increased hydrogen sulfide in produced waters (PW). Microbial community analysis by 16S rRNA gene sequencing showed the hyperthermophilic sulfate-reducing archaeon Archaeogl...

This short paper reviews two groups of enzymes designated as polyethylene terephthalate (PET) hydrolases: one consists thermophilic cutinases from microorganisms (actinomycetes and a fungus) the other mesophilic cutinases, representative which is IsPETase bacterium. From viewpoint that PET hydrolysis requires high temperature close to glass transition (65–70 °C in water) PET, are not suitable f...

The outer membrane of the thermophilic bacterium Thermus thermophilus was isolated using sucrose step gradient centrifugation. Its detergent extracts contained an ion-permeable channel with an extremely high single-channel conductance of 20 nS in 1 M KCl. The channel protein was purified by preparative sodium dodecyl sulfate (SDS)-polyacylamide gel electrophoresis. It has a high molecular mass ...