Three commercially available microbiology identification and susceptibility testing systems were compared with regard to their ability to detect extended-spectrum beta-lactamase (ESBL) production in Enterobacteriaceae, i.e., the Phoenix Automated Microbiology System (BD Diagnostic Systems, Sparks, MD), the VITEK 2 System (bioMérieux, Marcy l'Etoile, France), and the MicroScan WalkAway-96 System...

objective(s) this study was carried out to evaluate the existence of the tem, shv and per esbl genes in esbl producing strains of pseudomonas aeruginosa isolated from burnt patients at shafa-hospital, kerman, iran. materials and methods a total of 120 strains of p. aeruginosa were isolated from 245 patients in burn unit of shafa-hospital during january 2006 to december 2007. mic of antibiotics ...

OBJECTIVE This study is to define the statistical significance for detection of ESBL producers by the double disk synergy test and molecular test (Check-MDR CT102), microdilution test (VITEK 2 with AES) and double disk synergy test (DDST), as well as the microdilution test and molecular test. MATERIALS AND METHODS Phenotypic testing of 55 isolates Enterobacteriaceae (Escherichia coli (14/55),...

Enter obacteriaceae families are capable of producing the extended spectrum beta-lactamase (ESBL) enzyme, which results in highlevels resistance to all beta-lactam antibiotics. For effective antibiotic therapy, stop spread mechanisms, and forepidemiological reasons, their detection is crucial. 53 Gram negative isolates were detected using a standard methodology currentinvestigation, compared va...

BACKGROUND Antimicrobial resistance in hospital pathogens is an important concern. It can cause longer hospital stays, increase costs, and contribute to increased mortality and morbidity in hospitalized patients. The aim of this study was to categorize and identify gram-negative bacilli capable of ESBLs production and to study the effect of MIC silver nanoparticles on bacteria strains and then ...

BACKGROUND A common mechanism of resistance to beta-lactam antibiotics is the production of beta-lactamase by Gram-negative bacteria. Recently, nonderivative extended-spectrum beta-lactamases (ESBLs) from the TEM and SHV enzymes, such as CTX-M, that were related to different geographical regions have been recognized. OBJECTIVES The aim of this study was to determine the frequency of the CTX-M...

Of 72 blood isolates of Enterobacter spp. collected over an 8-year period, 50% (36 of 72) were derepressed or partially derepressed AmpC mutants. The extended-spectrum -lactamase (ESBL) production rate was 43% (31 of 72 isolates), and 67.3% (31 of 46) of extended-spectrum cephalosporin-resistant strains produced ESBLs. Thus, a confirmatory test for ESBL production is necessary for extended-spec...

In this study, 153 clinical isolates of Shigella were screened for extended-spectrum beta-lactamase (ESBL) production by double-disk synergy test. After being confirmed by the combined disk-test and inhibitor-combined E-test strips, all positive isolates were tested for the bla gene by PCR and the enzymes by isoelectric focusing. DNA sequencing of PCR products revealed that five Shigella sonnei...

The prevalence of Escherichia coli and Klebsiella pneumoniae that produce extended-spectrum b-lactamases (ESBL) was investigated in patients of a university hospital in Split, Croatia. Patients were grouped according to age (pediatric vs. adult), antibiotic type, and hospital ward. From Jan. 2001 to Dec. 2002, the susceptibility of E. coli and K. pneumoniae isolates to antimicrobials was tested...