FUS/TLS is an RNA/DNA-binding protein associated with neurodegenerative diseases including amyotrophic lateral sclerosis and frontotemporal lobar degeneration. Previously, we found that a prion-like domain in the N-terminus of FUS/TLS mediates co-aggregation between FUS/TLS and mutant huntingtin, the gene product of Huntington's disease (HD). Here, we show that heterozygous knockout of FUS/TLS ...

The use of TLS (translation/libration/screw) models to describe anisotropic displacement of atoms within a protein crystal structure has become increasingly common. These models may be used purely as an improved methodology for crystallographic refinement or as the basis for analyzing inter-domain and other large-scale motions implied by the crystal structure. In either case it is desirable to ...

Given a linear system Ax ≈ b over the real or complex field where both A and b are subject to noise, the total least squares (TLS) problem seeks to find a correction matrix and a correction righthand side vector of minimal norm which makes the linear system feasible. To avoid ill-posedness, a regularization term is added to the objective function; this leads to the so-called regularized TLS (RT...

A 1.10-A atomic resolution X-ray structure of human fibroblast growth factor 1 (FGF-1), a member of the beta-trefoil superfold, has been determined. The beta-trefoil is one of 10 fundamental protein superfolds and is the only superfold to exhibit 3-fold structural symmetry (comprising 3 "trefoil" units). The quality of the diffraction data permits unambiguous assignment of Asn, Gln, and His rot...

Different pathways of propagation and dispersal of non-native species into new environments may have contrasting demographic and genetic impacts on established populations. Repeated introductions of rainbow trout (Oncorhynchus mykiss) to Chile in South America, initially through stocking and later through aquaculture escapes, provide a unique setting to contrast these two pathways. Using a pane...

A wide variety of hard structures protect coastal activities and communities from the action tides waves worldwide. It is fundamental to monitor integrity structures, as interventions repairs may be needed in case damages. This work compares effectiveness an Unmanned Aerial System (UAS) a Terrestrial Laser Scanner (TLS) reproduce 3D geometry rocky groin. The Structure-from-Motion (SfM) photogra...

Although it is well known that spatial learning can be important in the biology of predators that actively move around in search for food, comparatively little is known about ways in which spatial learning might function in the strategies of sit-and-wait predators. In this study, Cyclosa octotuberculata, an orb-web spider that uses its legs to contract radial threads of its web to increase thre...

However it is possible to use of numerical methods such as beta-Newmark in order to investigate the structural response behavior of the dynamic systems under random sea wave loads but because of necessity to analysis the offshore systems for extensive time to fatigue study it is important to use of simple stable methods for numerical integration. The modified Euler method (MEM) is a simple nume...

To filter SSL/TLS-protected traffic, some antivirus and parental-control applications interpose a TLS proxy in the middle of the host’s communications. We set out to analyze such proxies as there are known problems in other (more matured) TLS processing engines, such as browsers and common TLS libraries. Compared to regular proxies, client-end TLS proxies impose several unique constraints, and ...