Bacteriophage phiX174 cannot grow in a temperature-sensitive dnaC mutant of Escherichia coli C at the nonpermissive temperature. The inability to grow is the result of inhibition of virus DNA synthesis. Parental replicative form synthesis is not temperature sensitive. Single-stranded virus DNA continues to be synthesized for at least 45 min after shifting to the nonpermissive temperature late i...

Most microorganisms isolated from low-temperature environments (below 4 degrees C) are eury-, not steno-, psychrophiles. While psychrophiles maximize or maintain growth yield at low temperatures to compensate for low growth rate, the mechanisms involved remain unknown, as does the strategy used by eurypsychrophiles to survive wide ranges of temperatures that include subzero temperatures. Our st...

The study identified and evaluated the options for population-level government policies to increase the prevalence of homes free of secondhand smoke (SHS). We used three criteria to evaluate the policy options: effectiveness, the reductions on inequalities in SHS exposure, and cost-effectiveness. Evidence indicates some association between relatively comprehensive tobacco control programmes and...

The goal of this work was to identify sequences encoding monooxygenase biocatalysts with novel features by in silico mining an assembled metagenomic dataset of polar and subpolar marine sediments. The targeted enzyme sequences were Baeyer-Villiger and bacterial cytochrome P450 monooxygenases (CYP153). These enzymes have wide-ranging applications, from the synthesis of steroids, antibiotics, myc...

The present study aims to analyze the psychometric properties of the Subjective Happiness Scale on a Romanian adult sample. The Romanian version of the SHS had excellent psychometric properties. All items loaded in the expected factor, being a unidimensional scale. The confirmatory factor analysis results revealed excellent fit indices. Convergent validity showed high associations with satisfac...

We examine the anyon representation of the Laughlin quasi-holes, in particular the onedimensional, algebraic aspects of the representation. For the cases of one and two quasiholes an explicit mapping to anyon systems is given, and the connection between the holestates and coherent states of the fundamental algebras of observables is examined. The quasi-electron case is discussed more briefly, a...

Smart home services (SHS) have been developed by related firms and have had a strong impact on consumers’ lives. This research integrates involvement antecedents, innovation diffusion factors and a technology acceptance model to develop a conceptual framework for discussing consumer attitudes and adoption behaviors toward smart home service applications. A sample of 580 respondents from differe...

This investigation tested the hypotheses that (1) individuals with higher levels of insight would be both happier and more satisfied with their lives than those with lower levels and (2) that an interaction between self-reflection and insight would affect both happiness and life satisfaction. Participants (N = 208) were community members who completed four self-report instruments. They were div...

In order to further understand the structural role of the modified nucleoside dihydrouridine in RNA the solution conformations of Dp and ApDpA were analyzed by one- and two-dimensional proton NRM spectroscopy and compared with those of the related uridine-containing compounds. The analyses indicate that dihydrouridine significantly destabilizes the C3'-endo sugar conformation associated with ba...