The cellular multiplicity of infection (MOI) is a key parameter for describing the interactions between virions and cells, predicting the dynamics of mixed-genotype infections, and understanding virus evolution. Two recent studies have reported in vivo MOI estimates for Tobacco mosaic virus (TMV) and Cauliflower mosaic virus (CaMV), using sophisticated approaches to measure the distribution of ...

Tobacco mosaic virus has been partially stripped of its protein coat by sodium dodecyl sulfate. The nucleic acid exposed was digested with T1 ribonuclease and the products were examined for the presence of three oligonucleotides of unique sequence. The results indicate the location of two of the unique oligomers to be near the 5’-OH-linked end and the third to be near the middle of tobacco mosa...

Tobacco is widely planted across the world especially in China, which means that a large amount of tobacco waste needs to be treated. This study investigated the biogas fermentation of tobacco stalks co-digested with different biomass feedstocks and the inactivation of Tobacco mosaic virus (TMV), Cucumber mosaic virus (CMV) by anaerobic digestion. Results showed that the maximum methane yield o...

Alfalfa mosaic virus (AlMV) coat protein is involved in systemic infection of host plants, and a specific mutation in this gene prevents the virus from moving into the upper uninoculated leaves. The coat protein also is required for different viral functions during early and late infection. To study the role of the coat protein in long-distance movement of AlMV independent of other vital functi...

Phenotypic expression of different genes controlling resistance to tobacco mosaic virus (TMV) in tomato was analysed in the protoplast system using otherwise isogenic breeding lines. Genes Tm-2 and Tin-22 were not expressed and did not prevent TMV-L, a common tomato strain of TMV, infecting and multiplying. By contrast, homozygous gene Tin-I was able to express its effect in protoplasts as well...

Normal and tobacco mosaic-diseased Turkish tobacco plants were grown in sand for a period of several weeks, during which they were fed daily a complete nutrient solution to which had been added disodium phosphate containing radioactive phosphorus. Determinations were made of the distribution of radioactive phosphorus in different fractions such as the wash from the sand and roots, the press cak...

Among seed-transmitted cereal viruses the most important is Barley stripe mosaic virus (BSMV), infecting barley. Investigations on the occurrence of BSMV in Poland have been carried out in the Institute of Plant Protection in Poznań since 2000. The studies aimed at the evaluation of the BSMV distribution in plant material, the rate of its seed transmission in different barley cultivars and the ...

Tobacco mosaic virus and turnip yellow mosaic virus genome RNAs, which have long (greater than or equal to 69 nucleotides) 5'-terminal leader sequences preceding the AUG initiation codon, can form disome initiation complexes when incubated with a wheat germ extract and sparsomycin. In tobacco mosaic virus RNA, whereas one ribosome in such complexes presumably occupies the AUG-containing initiat...