A polyphasic taxonomic approach was used to study atypical enterococci isolated from surface waters. All strains were characterized by physiological and biochemical tests as well as by genotyping. The results of biochemical tests and tRNA intergenic length polymorphism analysis (tDNA-PCR) divided all studied strains uniformly into two groups. Because these groups were clearly separated from all...

DNA metabarcoding is a promising approach for rapidly surveying biodiversity and is likely to become an important tool for measuring ecosystem responses to environmental change. Metabarcoding markers need sufficient taxonomic coverage to detect groups of interest, sufficient sequence divergence to resolve species, and will ideally indicate relative abundance of taxa present. We characterized zo...

numerical taxonomy and seed storage protein analysis of hyoscyamus species of iran was carried out with the aim to illustrate species inter-relationship and to check the sub-generic taxonomic treatment proposed for the genus. cluster analysis of morphological and protein data grouped the species in three separate clusters which supports the relationships of h. niger with h. reticulatus and h. k...

Ontologies have been widely accepted as the most advanced knowledge representation model. They are among the most important building blocks of semantic web, hence, very crucial for the success of semantic web. This paper discusses a fast and efficient method to facilitate the evaluation and enrichment of domain ontologies using a text-mining approach. We exploit domain specific texts and glossa...

The intestinal bacterial diversity of Stichopus japonicus was investigated using 16S ribosomal RNA gene (rDNA) clone library and Polymerase Chain Reaction/Denaturing Gradient Gel Electrophoresis (PCR-DGGE). The clone library yielded a total of 188 clones, and these were sequenced and classified into 106 operational taxonomic units (OTUs) with sequence similarity ranging from 88 to 100%. The cov...

during a survey of sindh and punjab provinces of pakistan, two dragonfly genera were collected and identified for the first time from the country. anaciaeshna(family aeshnidae) is a genus of large dragonflies. representatives were collectedfrom gujjo, district thatta (sindh) in august 2008 and were identified as anaciaeshna jaspidea (burmeister). the second genus was epophthalmia (family cordul...

Water discharging from abandoned coal mines can contain extremely high manganese levels. Removing this metal is an ongoing challenge. Passive Mn(II) removal beds (MRBs) contain microorganisms that oxidize soluble Mn(II) to insoluble Mn(III/IV) minerals, but system performance is unpredictable. Using amplicon pyrosequencing, we profiled the bacterial, fungal, algal, and archaeal communities in f...

The species /time relationship (STR) describes how the species richness of a community increases with the time span over which the community is observed. This pattern has numerous implications for both theory and conservation in much the same way as the species /area relationship (SAR). However, the STR has received much less attention and to date only a handful of papers have been published on...

Citation Shi, Yanmei et al. " Integrated metatranscriptomic and metagenomic analyses of stratified microbial assemblages in the open ocean. The MIT Faculty has made this article openly available. Please share how this access benefits you. Your story matters. Abstract 1 As part of an ongoing survey of microbial community gene expression in the 2 ocean, we sequenced and compared a total of ~38 Mb...