نتایج جستجو برای: taq man allele

تعداد نتایج: 259493  

2017
Khalid Meksem Eliza Ruben David L. Hyten Michael E. Schmidt David A. Lightfoot

An individual soybean breeder can generate over one hundred thousand new genotypes each year. The efficiency of selection in these populations could be improved if these genotypes were effectively screened with one DNA marker that identified an important gene, and if laboratory throughput was high and costs were low. Our aim was to develop a rapid genotyping procedure for resistance to the soyb...

Journal: :BioTechniques 2007
Jun Watanabe Yasumasa Mitani Yuki Kawai Takeshi Kikuchi Yasushi Kogo Atsuko Oguchi-Katayama Hajime Kanamori Kengo Usui Masayoshi Itoh Paul E Cizdziel Alexander Lezhava Kenji Tatsumi Yasushi Ichikawa Shinji Togo Hiroshi Shimada Yoshihide Hayashizaki

A key feature of the smart amplification process version 2 (SMAP-2) is the ability to suppress mismatch amplification by using a unique asymmetric primer design and Thermus aquaticus MutS (Taq MutS). However we report here that use of SMAP-2 for polymorphism determination of the UGT1A1 *28 allele required a further ancillary approach for complete background suppression. The UGT1A1 *28 allele is...

2008
Katharina Neureuther Thomas Langman Stephan Schneuwly Thomas Langmann Christian Hengstenberg

Association of single nucleotide polymorphisms in the LPA gene region with serum Lp(a) levels and myocardial infarction Dissertation zur Erlangung des Doktorgrades der Naturwissenschaften (Dr. rer. Quidquid agis prudenter agas et respice finem. AA amino acid apo(a) apolipoprotein (a) asPCR allele-specific PCR BMI body mass index bp base pair CAD coronary artery disease CHD coronary heart diseas...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1995
R Winchester Y Chen S Rose J Selby W Borkowsky

Transmission of human immunodeficiency virus 1 (HIV-1) from an infected women to her offspring during gestation and delivery was found to be influenced by the infant's major histocompatibility complex class II DRB1 alleles. Forty-six HIV-infected infants and 63 seroreverting infants, born with passively acquired anti-HIV antibodies but not becoming detectably infected, were typed by an automate...

Journal: :In vivo 2010
Kivanç Bektas-Kayhan Meral Unür Ilhan Yaylim-Eraltan H Arzu Ergen Bahar Toptas Günter Hafiz Ahmet Karadeniz Turgay Isbir

BACKGROUND It has been hypothesised that vitamin D receptor (VDR) gene polymorphisms may influence both the risk of cancer occurrence and prognosis. MATERIALS AND METHODS The distribution of VDR Taq I polymorphism in 64 patients with OSCC was determined by polymerase chain reaction based restriction fragment length polymorphism (RFLP) and compared with that of 87 healthy controls. RESULTS T...

Journal: :Forensic science international 2004
Peter M Schneider Klaus Bender Wolfgang R Mayr Walther Parson Bernadette Hoste Ronny Decorte Jan Cordonnier Daniel Vanek Niels Morling Matti Karjalainen C Marie-Paule Carlotti Myriam Sabatier Carsten Hohoff Hermann Schmitter Werner Pflug Rainer Wenzel Dieter Patzelt Rüdiger Lessig Peter Dobrowolski Geraldine O'Donnell Luciano Garafano Marina Dobosz Peter De Knijff Bente Mevag Ryszard Pawlowski Leonor Gusmão Maria Conceicao Vide Antonio Alonso Alonso Oscar García Fernández Pilar Sanz Nicolás Ann Kihlgreen Walter Bär Verena Meier Anne Teyssier Raphael Coquoz Conxita Brandt Ursula Germann Peter Gill Justine Hallett Matthew Greenhalgh

Degradation of human DNA extracted from forensic stains is, in most cases, the result of a natural process due to the exposure of the stain samples to the environment. Experiences with degraded DNA from casework samples show that every sample may exhibit different properties in this respect, and that it is difficult to systematically assess the performance of routinely used typing systems for t...

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