The fluorescence intensity of membrane-bound N-phenyl-1-naphthylamine increases dramatically when T5 bacteriophage infect colicin Ib plasmid-containing hosts. This dramatic increase is not seen during normal infections or in infections wherein either the plasmid or the phage contain mutations which allow productive infection to occur. Two smaller increases in fluorescence intensity are seen, ho...

The anatomy of DNA molecules isolated from mature bacteriophage is reviewed. These molecules are linear, duplex DNA consisting mainly of uninterrupted polynucleotide chains. Certain phage (T5 and PB) contain four specifically located interruptions. While the nucleotide sequence of most of these molecules is unique (T5, T3, T7, lambda), some are circular permutations of each other (T2, T4, P22)....

Methods. One hundred and sixteen patients with traumatic complete SCI were grouped according to the time elapsed after the injury (,3 days and .9 months) and the level of injury (above T5 and below T5): acute high (AH, n1⁄425), chronic high (CH, n1⁄426), acute low (AL, n1⁄420), and chronic low (CL, n1⁄445). Twenty-five patients without SCI served as a control group. Bispectral index (BIS) respo...

Se evaluó los parámetros de composición química, degradación y cinética ruminal del ensilado pasto elefante (Pennisetum purpureum) con inclusión residuos cascara maracuyá (Passiflora edulis). aplico un diseño bloques completos al azar cuatro tratamientos (T1:0%; T2:10%; T3:20% ,k 30% T4:40%) maracuyá, tres (bovinos rumen fistulados) siete tiempos incubación (0, 3, 6, 12, 24, 48 72 horas). Las d...

BACKGROUND This randomized, controlled study was designed to compare the effects of recruitment maneuvers (RMs) with a 15 cmH2O positive end-expiratory pressure (PEEP) on the systemic oxygenation and lung compliance of patients with healthy lungs following robot-assisted laparoscopic prostatectomy (RALP). METHODS Sixty patients undergoing a RALP with an intraoperative 15 cmH2O PEEP were rando...

BACKGROUND The effectiveness of conservative treatment of scoliosis is controversial. Some studies suggest that brace is effective in stopping curve progression, whilst others did not report such an effect.The purpose of the present study was to effectiveness of Progressive Action Short Brace (PASB) in the correction of thoraco-lumbar curves, in agreement with the Scoliosis Research Society (SR...

A comprehensive survey assessing the presence of Acanthamoeba was conducted on 50 samples from water sources in parks and public squares from 22 municipal districts of Tehran, Iran. The prevalence and genotypes of Acanthamoeba were determined by PCR and the PCR fragments of ribosomal RNA genes sequenced. Sixteen (32%) samples were positive for Acanthamoeba spp. Sequence analysis revealed that t...

Visual systems extract directional motion information from spatiotemporal luminance changes on the retina. An algorithmic model, the Reichardt detector, accounts for this by multiplying adjacent inputs after asymmetric temporal filtering. The outputs of two mirror-symmetrical units tuned to opposite directions are thought to be subtracted on the dendrites of wide-field motion-sensitive lobula p...

Sex determination in Drosophila depends on X-linked 'numerator' genes activating early Sex-lethal (Sxl) transcription in females. One numerator gene, sisterless-b (sis-b), corresponds to the achaete-scute (AS-C) T4 basic-helix-loop-helix (bHLH) gene. Two other closely related AS-C bHLH genes, T3 and T5, appear not to function as numerator elements. We analyzed endogenous AS-C expression and sho...