Trichoderma harzianum strain ESALQ-1306 was assessed for its potential biological control against Meloidogyne incognita race 4 under in vitro and greenhouse conditions. In vitro, we noticed 64% of conidial attachment on eggs and second-stage juvenile immobilized by the fungus. In greenhouse, cucumber plants were treated with different concentrations of conidial suspensions before and after inoc...

We have investigated the molecular basis for the reported synergism between peptaibols and cell wall hydrolytic enzymes in the antagonism of phytopathogenic fungi by Trichoderma harzianum. beta-Glucan synthase activity on isolated plasma membranes of Botrytis cinerea was inhibited in vitro by the peptaibols trichorzianin TA and TB, and this inhibition was reversed by the addition of phosphatidy...

Monoconidial cultures of 15 isolates of Trichoderma harzianum were characterized on the basis of 82 morphological, physiological, and biochemical features and 99 isoenzyme bands from seven enzyme systems. The results were subjected to numerical analysis which revealed four distinct groups. Representative sequences of the internal transcribed spacer 1 (ITS 1)-ITS 2 region in the ribosomal DNA ge...

Trichoderma harzianum is a filamentous fungus reported to be a producer of extracellular N-acetyl-beta-D-glucosaminidase (NAGase) when grown in chitin-containing medium. An approximately 64-kDa protein with NAGase activity was purified by gel filtration and ion exchange chromatography. The involvement of cyclic AMP (cAMP) in the synthesis of NAGase from T. harzianum in chitin-containing medium ...

We surveyed the Trichoderma (Hypocreales, Ascomycota) biodiversity in agricultural fields in four major agricultural provinces of East China. Trichoderma strains were identified based on molecular approaches and morphological characteristics. In three sampled seasons (spring, summer and autumn), 2078 strains were isolated and identified to 17 known species: T. harzianum (429 isolates), T. asper...

BACKGROUND Rice straw has shown to be a promising agricultural by-product in the bioconversion of biomass to value-added products. Hydrolysis of cellulose, a main constituent of lignocellulosic biomass, is a requirement for fermentable sugar production and its subsequent bioconversion to biofuels such as biobutanol. The high cost of commercial enzymes is a major impediment to the industrial app...

root-knot nematodes (meloidogyne spp.) are among the most important groups of plant-parasitic nematodes, causing damage and yield losses to most cultivated plants. the bacterium pseudomonas fluorescens cha0 and the fungus trichoderma harzianum bi were investigated for biological control capability and systemic induction of resistance against the nematode m. javanica under glasshouse conditions....

nine organic substrates viz., rice grains, sorghum grains, wheat grains, millet grains, wheat straw, rice husk, cow dung, sawdust, and poultry manure were used for mass multiplication of trichoderma harzianum. of these,sorghum grains followed by millet grains were the best substrates. the poultry manure appeared to be the most unsuitable substrate, whereas rice grains, wheat grains, wheat straw...

Nine fungicides namely: carbendazim, hexaconazole, thiophonate methyl, triadimefan, metalaxyl, mancozeb, captan, copper oxychloride and chlorothalonil and seven bioagents namely: Trichoderma harzianum, Trichoderma viride, Gliocladium virens, Lacaria laccata, Boletus edulis, Suillus placids and Russula lutea were evaluated in vitro by using dual culture and cultural filtrate techniques for their...