نتایج جستجو برای: synthetic promoter

تعداد نتایج: 228934  

Journal: :Cell systems 2017
Enoch Yeung Aaron J Dy Kyle B Martin Andrew H Ng Domitilla Del Vecchio James L Beck James J Collins Richard M Murray

Synthetic gene expression is highly sensitive to intragenic compositional context (promoter structure, spacing regions between promoter and coding sequences, and ribosome binding sites). However, much less is known about the effects of intergenic compositional context (spatial arrangement and orientation of entire genes on DNA) on expression levels in synthetic gene networks. We compare express...

Journal: :The Plant cell 1997
T Ulmasov J Murfett G Hagen T J Guilfoyle

A highly active synthetic auxin response element (AuxRE), referred to as DR5, was created by performing site-directed mutations in a natural composite AuxRE found in the soybean GH3 promoter. DR5 consisted of tandem direct repeats of 11 bp that included the auxin-responsive TGTCTC element. The DR5 AuxRE showed greater auxin responsiveness than a natural composite AuxRE and the GH3 promoter when...

2002
Tim Ulmasov Jane Murfett Gretchen Hagen Tom J. Guilfoyle

A highly active synthetic auxin response element (AuxRE), referred to as DR5, was created by petforming site-directed mutations in a natural composite AuxRE found in the soybean GH3 promoter. DR5 consisted of tandem direct repeats of 11 bp that included the auxin-responsive TGTCTC element. The DR5 AuxRE showed greater auxin responsiveness than a natural composite AuxRE and the GH3 promoter when...

2015
Kathleen Brückner Petra Schäfer Ernst Weber Ramona Grützner Sylvestre Marillonnet Alain Tissier

A library of synthetic promoters containing the binding site of a single designer transcription activator-like effector (dTALE) was constructed. The promoters contain a constant sequence, consisting of an 18-base long dTALE-binding site and a TATA box, flanked by degenerate sequences of 49 bases downstream and 19 bases upstream. Forty-three of these promoters were sequenced and tested in transi...

Journal: :Chemistry & biology 2014
Fuqing Wu David J Menn Xiao Wang

Widespread quorum-sensing (QS) enables bacteria to communicate and plays a critical role in controlling bacterial virulence. However, effects of promiscuous QS crosstalk and its implications for gene regulation and cell decision-making remain largely unknown. Here we systematically studied the crosstalk between LuxR/I and LasR/I systems and found that QS crosstalk can be dissected into signal c...

2013
David Bikard Wenyan Jiang Poulami Samai Ann Hochschild Feng Zhang Luciano A. Marraffini

The ability to artificially control transcription is essential both to the study of gene function and to the construction of synthetic gene networks with desired properties. Cas9 is an RNA-guided double-stranded DNA nuclease that participates in the CRISPR-Cas immune defense against prokaryotic viruses. We describe the use of a Cas9 nuclease mutant that retains DNA-binding activity and can be e...

Journal: :journal of agricultural science and technology 2014
a. a. masoudi

the aim of this study was to analyze the polymorphisms existing in the 5´ flanking region, exonic, and some parts of the intronic regions as well as methylation analysis of the ednrb gene in cattle and goat. these regions were sequenced in three different breeds of cattle including sistani, golpayegani, and holstein and were compared with that of marghoz goat. the results identified that this g...

Journal: :iranian journal of basic medical sciences 0
soudabeh javadian-elyaderani department of reproductive biotechnology, reproductive biomedicine research center, royan institute for biotechnology, acecr, isfahan, iran department of biology, science and research branch, islamic azad university, tehran, iran kamran ghaedi department of molecular biotechnology, cell science research center, royan institute for biotechnology, acecr, isfahan, iran biology department, school of sciences, university of isfahan, isfahan, iran marziyeh tavalaee department of reproductive biotechnology, reproductive biomedicine research center, royan institute for biotechnology, acecr, isfahan, iran farzaneh rabiee department of molecular biotechnology, cell science research center, royan institute for biotechnology, acecr, isfahan, iran

objective(s): phospholipase c ζ (plcζ) is considered as a nominee for sperm associated oocyte activating factors and is located back-to-back with capza3, an actin-capping protein controlling actin polymerization during spermiogenesis. they contain a common bidirectional promoter. the objective of this study was to identify individuals with parallel low expression of plcζ and capza3 mrna, in hop...

2014
David L. Shis Faiza Hussain Sarah Meinhardt Liskin Swint-Kruse Matthew R. Bennett

In prokaryotes, the construction of synthetic, multi-input promoters is constrained by the number of transcription factors that can simultaneously regulate a single promoter. This fundamental engineering constraint is an obstacle to synthetic biologists because it limits the computational capacity of engineered gene circuits. Here, we demonstrate that complex multi-input transcriptional logic g...

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