This discussion presumes that you already have an understanding of the basic theory of NMR. There are a number of issues that should be considered when measuring NMR spectra for quantitative analysis. Many of these issues pertain to the way that the NMR signal is acquired and processed. It is usually necessary to perform Q-NMR measurements with care to obtain accurate and precise quantitative r...

Human serum transferrin N-lobe (hTF/2N) contains three conserved tryptophan residues, Trp(8), Trp(128) and Trp(264), located in three different environments. The present report addresses the different contributions of the three tryptophan residues to the UV-visible, fluorescence and NMR spectra of hTF/2N and the effect of the mutations at each tryptophan residue on the iron-binding properties o...

This paper presents an exploratory study of the binding interactions of xenon with the surface of several different proteins in the solution and solid states using both conventional and hyperpolarized (129)Xe NMR. The generation of hyperpolarized (129)Xe by spin exchange optical pumping affords an enhancement by 3-4 orders of magnitude of its NMR signal. As a result, it is possible to observe X...

Variable-temperature hyperpolarized (HP) 129Xe NMR spectroscopy has been employed to characterize surface properties of mesoporous MCM-41 modified by silylation treatment. The characteristic chemical shifts responsible for Xe-surface interactions exhibit strong correlations with both the surface coverage and chain length of the grafted alkylsilanes. Consequently, the deshielding medium contribu...

(1)H and (13)C NMR spectra of allyl isothiocyanate (AITC) were measured, and the exchange dynamics were studied to explain the near-silence of the ITC carbon in (13)C NMR spectra. The dihedral angles α = ∠(C1-C2-C3-N4) and β = ∠(C2-C3-N4-C5) describe the conformational dynamics (conformation change), and the bond angles γ = ∠(C3-N4-C5) and ε = ∠(N4-C5-S6) dominate the molecular dynamics (confor...

Siglec-2 undergoes constitutive endocytosis and is a drug target for autoimmune diseases and B cell-derived malignancies, including hairy cell leukaemia, marginal zone lymphoma, chronic lymphocytic leukaemia and non-Hodgkin's lymphoma (NHL). An alternative to current antibody-based therapies is the use of liposomal nanoparticles loaded with cytotoxic drugs and decorated with Siglec-2 ligands. W...

H and C NMR spectra of allyl isothiocyanate (AITC) were measured, and the exchange dynamics were studied to explain the near-silence of the ITC carbon in C NMR spectra. The dihedral angles α = ∠(C1−C2−C3−N4) and β = ∠(C2−C3−N4−C5) describe the conformational dynamics (conformation change), and the bond angles γ = ∠(C3−N4−C5) and ε = ∠(N4−C5−S6) dominate the molecular dynamics (conformer flexibi...

Cellulose fibres were treated with sodium hydroxide (NaOH) to improve the accessibility of chemical reagents and the roughness of fibre surface at low temperatures. The accessibility, supermolecular structure, morphology, and physical properties of fibres were investigated using solid-state cross polarisation/magic angle spinning 13C nuclear magnetic resonance (CP/MAS 13C NMR), wide-angle X-ray...