نتایج جستجو برای: surface absorbance
تعداد نتایج: 643594 فیلتر نتایج به سال:
In recent years, fluorescence microscopy has been revolutionized. Reversible switching of fluorophores enabled circumventing the limits imposed by diffraction. Thus, resolution down to molecular scale became possible. However, best our knowledge, application principles underlying super-resolution reflection not experimentally demonstrated. Here, we present first evidence that this is indeed A l...
The preparation and observations of spheroplast W303 cells are described with Environmental Scanning Electron Microscope (ESEM). The spheroplasting conversion was successfully confirmed qualitatively, by the evaluation of the morphological change between the normal W303 cells and the spheroplast W303 cells, and quantitatively, by determining the spheroplast conversion percentage based on the OD...
We demonstrate how grating-coupler assisted infrared reflection absorption spectroscopy can be used to simultaneously determine the chemical identity and relative thickness of organic thin films. With a grating substrate, a threshold anomaly associated with passing off of the -1 diffracted order occurs at grazing angles of incidence, resulting in a sharp absorbance in the infrared. The position...
Absorbance signals were recorded from cut single skeletal muscle fibers stained with the nonpenetrating potentiometric dye NK2367 and mounted in a three-vaseline-gap voltage clamp . The characteristics of the optical signals recorded under current and voltage-clamp conditions were studied at various wavelengths between 500 and 800 nm using unpolarized light . Our results indicate that the absor...
Focusing on the use of planar waveguides as platforms for highly sensitive attenuated total reflection spectroscopy of organic thin films, we extend the ray optics model to provide absorbance expressions for the case of dichroic layers immobilized on the waveguide surface. Straightforward expressions are derived for the limiting case of weakly absorbing, anisotropically oriented molecules in th...
Two-photon initiated photo-isomerization of an azobenzene moiety adsorbed on silver nanoparticles (Ag NPs) is demonstrated. The azobenzene is linked to a materials-binding peptide that brings it into intimate contact with the Ag NP surface, producing a dramatic enhancement of its two-photon absorbance. An integrated modeling approach, combining advanced conformational sampling with Quantum Mech...
A new ligand that covalently attaches to the surface of colloidal CdSe/CdS nanorods and can simultaneously chelate a molecular metal center is described. The dithiocarbamate-bipyridine ligand system facilitates hole transfer through energetic overlap at the inorganic-organic interface and conjugation through the organic ligand to a chelated metal center. Density functional theory calculations s...
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