Bovine thyroglobulin was treated with increasing ratios of succinic anhydride, trinitrobenzene sulfonic acid, tetranitromethane, and N-acetylimidazole in an attempt to assess the role of lysine or tyrosine residues in binding to thyroid membrane receptors. Extensive succinylation results in dissociation to 12 S thyroglobulin with retention of a considerable portion of the three-dimensional stru...

Materials The following materials were purchased from Sigma-Aldrich: sulfuric acid (H2SO4), hydrogen peroxide solution (H2O2), (3-aminopropyl)triethoxysilane (APTES), N-(3-dimethylaminopropyl)-N′ethylcarbodiimide (EDC), N-hydroxysuccinimide (NHS), succinic anhydride (SA), magnesium acetate solution, dimethyl sulfoxide (DMSO), triethylamine (TEA), and tris-EDTA buffer solution. The following DNA...

BACKGROUND We report on aminopropyltriethoxysilane (APTS)-mediated surface modification of nanohydroxyapatite with different surface functional groups for potential biomedical applications. In this study, nanohydroxyapatite covalently linked with APTS (n-HA-APTS) was reacted with acetic anhydride or succinic anhydride to produce neutralized (n-HA-APTS. Ac) or negatively charged (n-HA-APTS.SAH) ...

The synthesis and characterization of second- and third-generation triazine dendrimers bearing carboxylic acid groups on the periphery are reported. These materials were synthesized by exhaustive succinylation of amine-terminated dendrimers. (1)H and (13)C NMR spectra are consistent with the desired products, but these techniques are limited by degeneracy in signals. MALDI-TOF mass spectrometry...

The ability of the scavenger receptor of human monocyte macrophages to recognize human low density lipoproteins (LDL) progressively modified by three lysine-specific reagents, malondialdehyde, acetic anhydride, or succinic anhydride, has been investigated. Regardless of the reagent utilized, receptor-mediated uptake was dependent upon modification of greater than 16% of the peptidyl lysines rat...

A suitable formulation to improve Lavandin essential oil applicability as biocide has been study in this project. Agrochemical formulations are required to be long-term physical stable and enhance biological performance of the agrochemical. Particle encapsulation of lavandin oil had been considered as possible formulation. Two high pressure spray techniques (PGSS and PGSS drying) had been appli...

Octenyl succinic anhydride (OSA) modified early Indica rice starch was prepared in aqueous slurry systems using response surface methodology. The paste properties of the OSA starch were also investigated. Results indicated that the suitable parameters for the preparation of OSA starch from early Indica rice starch were as follows: reaction period 4 h, reaction temperature 33.4 °C, pH of reactio...

In this paper we describe the development and the evaluation of a new type of immunoassay for human corticotropin (ACTH). We succeeded, by using an original approach based upon immunization with ACTH derivatized with succinic anhydride, in raising monoclonal antibodies against this poorly immunogenic peptide. Three of the antibodies were selected to develop an immunoassay for ACTH. The assay re...

1. Studies on the inactivation of rat intestinal alkaline phosphatase by several metal-binding agents, namely EDTA, 8-hydroxyquinoline, pyridine-2,6-dicarboxylic acid, aoc'-bipyridyl, o-phenanthroline and sodium cyanide, indicated the functional role of a metal, probably zinc, in the catalysis. The metal ligands lowered stereospecific uncompetitive inhibition of the enzyme by L-phenylalanine by...

Succinyl phosphate reacts rapidly and nonenzymatically with coenzyme A in the pH range 3 to 8 to yield succinyl coenzyme A. This hitherto unsuspected reaction of an acyl phosphate with a thiol depends critically on the presence within the molecule of a free carboxyl group, which bears a “succinyl” relationship-and is spatially accessible-to the phosphorylated carboxyl. The free carboxyl group i...