The effect of organic and inorganic nitrogen sources on Trichoderma reesei Rut-C30 cellulase production was investigated in submerged cultivations. Stirred tank bioreactors and shake flasks, with and without pH control, respectively, were employed. The experimental design involved the addition of individual organic nitrogen sources (soy peptone, glutamate, glycine and alanine) within a basal me...

the acute coronary syndrome due to the left main coronary artery (lmca) thrombosis is a clinically rare and catastrophic event.   we describe a young man ( smoker, alcoholic, and drug abuser) with a history of recent surgery and typical chest pain who had non-occlusive lmca thrombosis in coronary angiography. the thrombosis was successfully treated with two 180 µ/kg intracoronary boluses of ept...

In the present work, different methods were tested to evaluate the adsorption and desorption of metalaxyl in two acid soils with different organic carbon and clay contents. The three methods (batch, stirred flow chamber and column) that were examined produced similar findings when the two soils were compared: (a) the metalaxyl adsorption capacity was higher in the soil with higher organic matte...

In this work, guidelines for reliable 3D-PTV measurements of fluid flows in stirred vessels at the lab-scale have been developed. The flow water Re=12,000 a flat bottom cylindrical tank (T=180mm) with 6 blades Rushton turbine (c=D=T/3) has measured different camera frame rates (125 – 3600 fps) and tracer concentrations (0.001 0.010 px?2). best compromise between number data, tracking efficiency...

Driven by global competition and rising cost pressure in the pharmaceutical industry, over the last ten years single-use bioreactors have been increasingly used for animal cell cultivations in screening experiments, seed inoculum and seed train productions as well as in small and medium scale production processes of proteins (in particular, antibodies and vaccines). In contrast to re-usable bio...

A novel milliliter-scale stirred tank bioreactor was developed for the cultivation of mycelium forming microorganisms on a 10 milliliter-scale. A newly designed one-sided paddle impeller is driven magnetically and rotates freely on an axis in an unbaffled reaction vessel made of polystyrene. A rotating lamella is formed which spreads out along the reactor wall. Thus an enhanced surface-to-volum...

Pyrolysis of low density polyethylene (LDPE) by equilibrium fluid catalytic cracking (FCC) was studied in a stirred reactor under different process parameters. In this work, the effect of process parameters such as degradation temperature (420-510°C), catalyst/polymer ratio (0-60%), carrier gas type (H2, N2, ethylene, propylene, Ar and He), residence time and agitator speed (0-300 rpm) on the c...

Chiral symmetry breaking in stirred crystallization of sodium chlorate (NaCI03) is believed to occur via nucleation of a single chiral "mother crystal" that produces a new generation of crystals which inherit its chirality. We here report results of stirred crystallizations resulting in massive "instantaneous" primary nucleation and total symmetry breaking. The results of these experiments sugg...