Anaerobic threshold is widely used for diagnosis of swimming aerobic endurance but the precise incremental protocols step duration for its assessment is controversial. A physiological and biomechanical comparison between intermittent incremental protocols with different step lengths and a maximal lactate steady state (MLSS) test was conducted. 17 swimmers performed 7×200, 300 and 400 m (30 s an...

This work describes jump-to-contact STM-break junction experiments leading to novel statistical distribution of last-step length associated with conductance of a single atom contact. Last-step length histograms are observed with up to five for Fe and three for Cu peaks at integral multiples close to 0.075 nm, a subatomic distance. A model is proposed in terms of gliding from a fcc hollow-site t...

The highly processive motor, myosin V, has an extremely long neck containing six calmodulin-binding IQ motifs that allows it to take multiple 36 nm steps corresponding to the pseudo-repeat of actin. To further investigate how myosin V moves processively on actin filaments, we altered the length of the neck by adding or deleting IQ motifs in myosin constructs lacking the globular tail domain. Th...

Parkinson’s disease (PD) is associated with several changes in motor tasks including postural control and gait. As such, patients with PD are more likely to experience a fall than their aged-matched peers. In fact, 80 percent of patients with PD fall during the course of their disease with 30 percent falling weekly. Recent research suggests gait variability may be an important predictor of both...

The full-length human androgen receptor with an N-terminal biotin acceptor peptide tag was overexpressed in Spodoptera frugiperda cells in the presence of 1 microM dihydrotestosterone. Site-specific biotinylation of BAP was achieved in vivo by co-expression of E. coli biotin holoenzyme synthetase. The androgen receptor was purified by single-step affinity chromatography using Streptavidin Mutei...

BACKGROUND In hemiplegic gait, step length typically differs in magnitude between paretic and nonparetic sides. However, the direction of step-length asymmetry varies across stroke patients. OBJECTIVE The study sought to understand directional variations in step-length asymmetry in terms of asymmetries in forward foot placement relative to the trunk and trunk progression. METHODS A total of...

To travel on rough terrain, a legged system must use the available footholds, even when they are isolated or hidden by obstacles. This thesis addresses the task of adjusting the length of each step to place the feet on available footholds, in the context of a dynamic biped robot that actively balances itself as it runs. In order for the biped to use specific footholds, the control system must s...

When increasing ambulation speed in Parkinson's disease, step cadence increases more than stride length, indicating movement scaling difficulties that affect step generation in particular. We investigated whether step length variation when increasing ambulation speed was related to disease progression. Patients with Parkinson's disease (N = 39) and controls (N = 152) performed two timed ambulat...

when the degree of saturation at intersection approaches one, webster’s optimum cycle length equation becomes inapplicable, because the cycle length will becomes very big when the degree of saturation approaches one and will be fully unrealistic when the degree of saturation becomes greater than one. this is not a problem for hcm2000 method. but optimum cycle length calculation in this method h...