Pathological diagnosis relies on the visual inspection of histologically stained thin tissue specimens, where different types stains are applied to bring contrast and highlight various desired histological features. However, destructive histochemical staining procedures usually irreversible, making it very difficult obtain multiple same section. Here, we demonstrate a virtual stain transfer fra...

Pathology is practiced by visual inspection of histochemically stained slides. Most commonly, the hematoxylin and eosin (H&E) stain used in diagnostic workflow it gold standard for cancer diagnosis. However, many cases, especially non-neoplastic diseases, additional "special stains" are to provide different levels contrast color tissue components allow pathologists get a clearer picture. In thi...

From the time the Leishman stain has been used for staining blood cells and parasites, it has been dissolved in acetone-free good quality methyl alcohol. Dissolving the stain in inferiorquality methyl alcohol or ethyl alcohol always gives poor results and hsematologists and parasitologists requiring precision work would always use acetone-free methyl alcohol of the best quality. After the secon...

A fluorescent nucleic acid stain that does not penetrate living cells was used to assess the integrity of the plasma membranes of bacteria. SYTOX Green nucleic acid stain is an unsymmetrical cyanine dye with three positive charges that is completely excluded from live eukaryotic and prokaryotic cells. Binding of SYTOX Green stain to nucleic acids resulted in a > 500-fold enhancement in fluoresc...