Conditions for accurate measurement of catalytic activity of aspartate aminotransferase and alanine aminotransferase in human serum have been reinvestigated. The basic variables (kind of buffer, buffer concentration, pH, ion effects, and the influence of pyridoxal-5-phosphate) can now be considered optimized. On this basis, the kinetic parameters of both aminotransferases were determined, i.e.,...

the activity of aspartate aminotransferase (ast) in human serum has been widely determined as a diagnostic aid in liver disease. in this study, the effect of aluminium on ast isoenzymes in relation to aluminium intoxified patients has been investigated. using gel filtration chromatography technique with sephacryl s-300, mitochondrial aminotransferase (m-ast) and cytosolic aminotransferase (c-as...

t-2 toxin, a trichothecene mycotoxin, is considered to be one of the most toxic compounds that are produced by molds, particularly the fusarium species. fusarium species have been recognized as a great agricultural problem. they occur worldwide on a variety of plant hosts and cereal grains. the aim of this study was to investigate t-2 toxin-induced liver injury using in situ perfused rat liver....

The influence of pyridoxal-5'-phosphate on the activities of alanine aminotransferase and aspartate aminotransferase from commercial test sera was investigated. Enhancement of the activity of alanine aminotransferase by the coenzyme is about 20% when 0.1 mmol/1 of pyridoxal-S'-phosphate is added to the reaction medium. This concentration seems sufficient for full saturation of the apoenzyme. Fo...

A series of novel (1-aminoalkyl)(trifluoromethyl)- and -(difluoromethyl)phosphinic acids - analogues of proteinogenic and nonproteinogenic α-amino acids were prepared. The synthetic methodology was based on nucleophilic addition of (trifluoromethyl)phosphinic acid or (difluoromethyl)phosphinic acid or its ethyl ester to substrates with C=N or activated C=C double bonds. Analogues of glycine, ph...

Administration of cortisol to rats enhanced brain tyrosine:2-oxoglutarate aminotransferase activity by a mechanism excluding stabilization, but possibly involving enhanced synthesis, of the enzyme.

PURPOSE To review the medical literature regarding the histopathologic and biochemical liver test abnormalities in chronic asymptomatic or oligosymptomatic alcoholics. METHODS Review of articles in the MEDLINE and LILACS databases regarding serum levels and prevalence of alterations in aspartate-aminotransferase, alanine-aminotransferase, alkaline phosphatase, and total bilirubin, in relation...

Regulation of tyrosine aminotransferase gene expression was studied in an adult rat hepatocyte line (RALA255-10G) which is temperature sensitive for the maintenance of the differentiated liver phenotype. Glucocorticoid hormones such as cortisol were necessary for expression of the aminotransferase gene. In the absence of these steroids, enzyme synthesis, activity, and mRNA accumulation were vir...