Two recent studies have demonstrated that clotrimazole, a well-known potential antifungal agent, inhibits the in vitro growth of chloroquine-resistant strains of the malaria parasite, Plasmodium falciparum. In a previous study, we suggested that clotrimazole acts as an anti-malarial agent by inhibiting heme catabolism in the malaria parasite and by enhancing heme-induced membrane damage. In thi...

We examined the effects of the angiotensin converting enzyme (ACE) inhibitors captopril, enalaprilat, quinapril, and trandolapril, and their active metabolites quinaprilat and trandolaprilat, on hemolysis induced by lysophosphatidylcholine (LPC) in human erythrocytes. LPC induced hemolysis at the concentrations above the critical micelle concentration (4 microM). Propranolol, used as a referenc...

When normal oxygenated blood is diluted 1 in 20 in a series of glucose solutions of different concentrations at 20°C., hemolysis does not begin until the concentration is reduced to about 2.2 gin. per 100 cc. and is complete when the concentration is further reduced to about 1.2 gm. per 100 cc. No standard series of osmotic hemolysis curves in glucose solutions has been carried out; suffice it ...

Both hypochlorite and ionizing radiation induce oxidation processes of biomolecules. The effects are dependent to a large degree on the dose of the oxidizing agent. Previously we observed that split doses of gamma radiation caused lower hemolysis than the same but single doses. The critical factors influencing the occurrence of this effect were: the value of the first dose and the time between ...

There has been described a type of hemolysis which occurs under certain defined conditions when erythrocytes are suspended in glucose solution. It consists of a prolytic phase lasting about an hour, followed by a hemolytic phase lasting about 2 hours. The physical factors controlling this delayed hemolysis have been investigated. The system is especially sensitive to changes of pH and of temper...

OBJECTIVE Establish whether hemolysis in samples collected from intravenous lines is influenced by catheterization site. METHODS Blood was collected from all patients (67 total) admitted to the emergency department the same morning, through a 20-gauge catheter placed in a vein of the upper limb directly into an evacuated blood tube. Serum was tested for hemolysis index by multi-wavelength pho...

The photodynamic action of riboflavin is generally considered to involve the generation of reactive oxygen species, whose production is enhanced when Cu(II) is present in the reaction. In the present study we report that photoactivated riboflavin causes K(+) loss from fresh human red blood cells (RBC) in a time dependent manner. Addition of Cu(II) further enhances the K(+) loss and also leads t...

Incubation of a 0.5% suspension of washed normal mouse erythrocytes with ferriprotoporphyrin IX (FP) for 2.5 h at 37 degrees C and pH 7.4 results in sufficient membrane damage to produce hemolysis. A sigmoidal dose-response curve is followed with 50% hemolysis being produced by 4 microM FP. Complete hemolysis is produced by 6 microM FP. The hemolytic process has at least two phases: a lag phase...

It is shown that photodynamic hemolysis may occur at -79 degrees C. if the erythrocytes are suspended in a solution containing 70 per cent glycerol which prevents hemolysis by freezing; but that there is no hemolysis under the same conditions at -210 degrees C. At the higher temperature the viscosity of the solution is still low enough to permit appreciable movement of molecules, whereas at the...