AIMS To detect clonal T cell populations by high resolution polymerase chain reaction (PCR) using fluorescently labelled nucleotides and analysis on an ABI 377 DNA sequencer, and to evaluate this method using low ionic strength single strand conformation polymorphism (LIS-SSCP) analysis. METHODS DNA samples from 11 patients diagnosed with a T cell disease and 15 with no known T cell disorder ...

The Nramp1 (Natural resistances macrophage protein1) gene or recently renamed Slc11a1 (solute carrier family 11 member1) is a member of a large family of genes coding for metal ion-transporting proteins. Nramp1 gene plays a critical role in innate immunity favoring bacterial killing by macrophages in addition to its influence on adaptative immunity. The aim of the present investigation was to i...

A fluorescence-based method for polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis, F-SSCP, was developed in which the target sequence is amplified by the PCR using fluorescent primers. The amplified products are then heat-denatured and applied to a water-jacket controlled gel in an automated DNA sequencer. The separated strands are detected as laser-excited f...

Periodontitis has been associated with the presence of Porphyromonas gingivalis, and previous studies have shown phenotypic differences in the pathogenicities of strains of P. gingivalis. An accurate and comprehensive phylogeny of strains of P. gingivalis would be useful in determining if there is an evolutionary basis to pathogenicity in this species. Previous phylogenies of P. gingivalis stra...

Either PCR-mediated single strand conformation polymorphism (SSCP) analysis or DNA sequencing of rpoB DNA (157 bp) can be used as a rapid screening method for the detection of mutations related to the rifampin resistance of Mycobacterium tuberculosis. However, due to the nonspecific amplification of rpoB DNA from nontuberculous mycobacteria these methods cannot be directly applied to clinical s...

The genetic heterogeneity of the spirochete Borrelia burgdorferi within single adult black-legged ticks from Shelter Island, N.Y., was determined by cold, single-strand conformation polymorphism (SSCP) analysis. The central region of the ospA gene of B. burgdorferi from infected ticks was amplified by nested PCR. Amplified product of the correct size was obtained from 20 to 45 ticks (44%). This...

The genomes of temperate Salmonella typhimurium phages P22 and L share approximately 69% homology, as measured by DNA heteroduplex analysis. Alignment of the P22/L heteroduplex molecules with a P22 physical map places most of this homology between the capsid genes and genes in the vicinity of the prophage attachment sites. The degree of genetic relatedness between these phages and the lambdoid ...

Applications of various molecular methods for the analysis of mutations in the @-globin gene has led to the identification of nearly 100 different @-thalassemia mutations in the world.’ Recently, two methods have been described for mutation detection that rely on the analysis of single-stranded conformation polymorphisms (sscp) ofDNA2 or of RNA.’ We find that DNA s s c p and RNA sscp analysis b...

The advent of the novel coronavirus, also called COVID-19, caused widespread disruptions worldwide. This unprecedented health crisis led to major in supply chain networks; therefore, innovative strategies and capabilities have potential alleviate its impacts. Considering this situation, study examined how COVID-19 impacted management innovation, information processing capability, business model...

We describe an improved, fast, automated method for screening large genes such as BRCA2 for germline genomic mutations. The method is based on heteroduplex analysis, and has been adapted for a high throughput application by combining the fluorescent technology of automated sequencers and robotic sample handling. This novel approach allows the entire BRCA2 gene to be screened with appropriate ov...