Real-time RT-PCR is used as a gold standard method for the diagnosis of COVID-19. Since real-time RT PCR nucleic acid-based, it highly sensitive and specific test. However, this test takes 4–8 h to generate results and, in emergency settings, delay may prove fatal certain patients. The frequent surge COVID cases increases patient load settings. Thus, acid-based rapid POC required that can quick...

abstract beet yellows virus (byv), the type member of the genus closterovirus and one of the most economically important yellowing viruses, causes serious losses in sugarbeet. in order to study infection and distribution of beet yellows virus during summer 2007, about 530 samples were collected from beet’s fields in razavi khorasan province (fariman, torbate jam, ghoochan, chenaran, torbate hey...

background: p19 cells are mouse embryonic carcinoma cells which contain pluripotent ability, like stem cells, to differentiate into different cell lines. there are several properties for this cell line that make it a valuable cell model for study of developmental stages. methods: at the first step, pts2-egfp coding sequence which was cloned in pucd2.hygro vector was used for transfection in to ...

asiatic citrus canker is a devastating disease resulting in drastic economic losses in citriculture worldwide. amongst three different types of the disease, i.e. a, a* and aw, the a* type is genetically less known. in order to comprehend the behavior of the asiatic citrus canker a*-type strain (xanthomonas citri subsp. citri) in the vicinity of the host cells, a targeted semi-quantitative trans...

zucchini yellow mosaic virus (zymv) is a potyvirus with a worldwide distribution. this virus causes serious economic losses in iran in many cucurbits. during 2002-2003, sam-ples were collected from squash fields in tehran province. five isolates (z1, z2, z3, z4 and z5) were inoculated on 27 species of cucurbitaceae, chenopodiaceae, amarantha-ceae,solanacae, leguminosae and ranunculaceae. chenop...

Infectious bursal disease (IBD) is an immunosuppressive, acute and highly contagious illness of growing-poultry stock infected with infectious bursal disease virus (IBDV). It is common in Pakistan, causing potential economic losses throughout the year. The objective of the study is to propose a rapid, sensitive and specific diagnostic tool, and compare it with existing commonly used reverse tra...

during 2010-2011, real-time pcr procedure was used to detecting fmdv rna on 147 epithelium samples from the field. in this survey, for real-time pcr from 3d gene segment as conserve region selected for tracking all of seven serotypes fmdv. the assay detected the viral rna in all serotypes of fmdv. the rrt-pcr specifically detected fmd virus in sample with greater sensitivity than our convention...