OBJECTIVE To evaluate the feasibility of tracking polyethylenimine (PEI)-wrapped superparamagnetic iron oxide (SPIO) nanoparticle-labeled, bone marrow-derived mesenchymal stem cells (BMSCs) by in vivo magnetic resonance imaging (MRI) in articular cartilage repair in a minipig model. METHODS Eighteen Guizhou minipigs were randomly divided into three groups (groups A, B, and C). In group A, PEI...

Schwann cell (SC) and olfactory ensheathing cell (OEC) transplantation has been shown experimentally to promote CNS axonal regeneration and remyelination. To advance this technique into a clinical setting it is important to be able to follow the fates of transplanted cells by noninvasive imaging. Previous studies, using complex modification processes to enable uptake of contrast agents, have sh...

OBJECTIVES To use the superparamagnetic iron oxide (SPIO) contrast agent Resovist (±transfection agent) to label human melanoma cells and determine its effects on cellular viability, microstructure, iron quantity, and magnetic resonance imaging (MRI) detectability. MATERIALS AND METHODS Human SK-Mel28 melanoma cells were incubated with Resovist (±liposomal transfection agent DOSPER). The cell...

Purpose: Superparamagnetic iron oxide (SPIO) nanoparticles are efficient contrast agents for molecular and cellular MR imaging [1]. They are usually considered to be negative contrast agents, since their magnetic moment causes signal dephasing which results in signal voids on T2* weighted images. However SPIO agents also have intrinsic T1 shortening properties [2] that can induce signal enhance...

This study determines the effect of naked and heparinbased super-paramagnetic iron oxide nanoparticles on the human cancer cell lines of A2780. Doxorubicin was used as the anticancer drug, entrapped in the SPIO-NPs. This study aimed to decorate nanoparticles with heparin, a molecular ligand for ‘active’ targeting of cancerous cells and the application of modified-nanoparticles in cancer treatme...

OBJECTIVE To evaluate engraftment by visualizing the location of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) three-dimensionally in photothrombotic cerebral infarction (PTCI) models of rats. MATERIALS AND METHODS Magnetic resonance imaging (MRI) of an agarose block containing superparamagnetic iron oxide (SPIO)-labeled hBM-MSCs was performed using a 3.0-T MRI, T2-(T2WI), T2(*)...

PURPOSE To determine the effects on arterial blood pressure and cerebral blood flow of intravenous bolus injection of three MR contrast agents: gadopentetate dimeglumine, polylysine-Gd-DTPA, and superparamagnetic iron particles (SPIO). METHODS A single-fiber laser Doppler flowmetry probe was placed intracerebrally in 56 anesthetized rats. Cerebral blood flow and mean arterial blood pressure w...

While having tremendous potential as therapeutic and imaging tools, the clinical use of engineered nanoparticles has been associated with serious safety concerns. Activation of the complement cascade and the release of proinflammatory factors C3a and C5a may contribute to infusion-related reactions, whereas opsonization with C3 fragments promotes rapid recognition and clearance of nanomaterials...

A multifunctional albumin/superparamagnetic iron oxide nanoparticle (SPIO) nanocomplex system to deliver IR780, a photothermal agent, for cancer theranostic applications was proposed in this study. Single emulsion method was utilized to fabricate the human albumin/IR780/SPIO (HISP) nanocomplexes with a hydrophobic core (SPIO and IR780) and a hydrophilic shell (human serum albumin (HSA) and poly...

Development of multifunctional nanoparticles that selectively target to human tumors for in vivo tumor imaging as well as inhibition of tumor growth holds a great promise for improving survival rate of cancer patients. In this study, we have engineered peptide conjugated superparamagnetic iron oxide (SPIO) nanoparticles targeting to urokinase plasminogen activator receptor (uPAR) or a single ch...