The recombinant antibodies AS739, AT693 and AU734 detect by immunofluorescence the spike S protein from SARS-CoV-2.

Avian infectious bronchitis is an acute and highly contagious disease that mainly causes respiratory symptoms in poultry. A number of serotypes and variants of the viral agent with poor cross-protection are the major problem to achieve desired immunity from vaccination. The S1 subunit of S glycoprotein (spike) is the major determinant of IBV so that a minor change in amino acid sequence of this...

hepatitis b virus (hbv) dna polymerase transactivated protein 1 (hbvdnaptp1) is a novel protein transactivated by hbv dna polymerase, screened by suppression subtractive hybridization technique (genbank accession no: ay450389). the biological function of hbvdnaptp1 was investigated in this study.we constructed a vector pcdna3.1 (-)/myc-his a-hbvdnaptp1 and used it to transfect acute monocytic l...

plant fatty acyl-acyl carrier protein thioesterase (fat) is a major enzyme regulating the amount and composition of fatty acids in lipids. in this study, one type of cdna, corresponding to the fatty acyl-acyl carrier protein (acyl-acp) thioesterase (fat) enzyme, was isolated from the seed of brassica napus cv. ningyou12. blast results revealed that the cdna identified highly with the fatb class...

background: there are only four drugs for treating african trypanosomiasis, a devastating disease in sub-saharan africa. with slow discovery of better drugs, vaccination is viewed as the best method of control. we previously showed that antibodies to native trypanosoma brucei brucei tubulin inhibit the growth of trypanosomes in culture. here, we aimed to determine the effect of antibodies to ba...

background the importance of sialic acid binding adhesin (saba) as a new outer membrane protein in gastroduodenal diseases has been recognized. the prevalence rate of saba gene varies in different geographic areas. objectives the aim of this study was to determine the frequency of saba gene in helicobacter pylori (h. pylori) strains isolated from different clinical outcomes in tehran, iran. pat...

Increased seed production has been a common goal during the domestication of cereal crops, and early cultivators of barley (Hordeum vulgare ssp. vulgare) selected a phenotype with a six-rowed spike that stably produced three times the usual grain number. This improved yield established barley as a founder crop for the Near Eastern Neolithic civilization. The barley spike has one central and two...

conclusions the gp40/15 gene, which was cloned in the pet28a+, was successfully expressed and produced in e. coli. therefore, this protein can be used in future studies to develop recombinant vaccines and diagnostic kits. methods in this experimental study, the gp40/15 gene sequence was extracted from genbank (no. af155624) and cloned in the pet28a+ plasmid. colony polymerase chain reaction (pc...

The recombinant antibodies AS739, AT693, AU197 and AU734 detect by western blot the spike S protein from SARS-CoV-2.