The aim of this investigation was to evaluate the inhibitory effect of ethanol extract of Lepidagathis alopecuroides on spermatogenesis in mammalian system. Thirty-six adult male Sprague-Dawley rats were used for this investigation and were divided into six groups of six rats each (A-F). Groups B-F were administered ethanol extracts of L. alopecuroides orally at a daily dose of 50-250 mg/kg bod...

The nuage is a germ cell-specific organelle that has been studied for more than a hundred years. It was first discovered in spermatogenic cells as a perinuclear granule stained by basic dyes and visualized using a light microscope. Morphological studies using electron microscopes demonstrated that ”chromatoid body (CB)” is a nuage component in spermatids, and that “inter-mitochondrial cement (I...

Testicular histopathology is considered the most sensitive and reliable method to detect effects of chemicals on sperm production. To carry out a examination testicular interpret changes require knowledge spermatogenic stages. Spermatogenic staging based acrosome development during spermiogenesis conventionally performed in animal species routinely used for research toxicity testing. In contras...

background: nanoparticles have wide range of application while there are some reports regarding their probable effects on male reproductive system and spermatozoa. objective:  the aim of this study was to evaluate the effect of different doses of silver nanoparticles (agnps) (70nm) on acrosome of rat spermatozoa and number of spermatogenic cells. materials and methods:  in this experimental stu...

The Sertoli cell is a critical somatic that initiates the development of testicular morphology and determines important parameters for spermatogenic function. blood-testis barrier, also known as barrier one tightest tissue barriers in mammalian body, an immunological to separate post meiotic germ antigens from systemic circulation. Additionally, creating unique microenvironment spermatocytes ex...

The monoclonal antibody (MAb), named TSd-1, specific to spermatogenic cells of the common tree shrew (Tupaia glis) was established and characterized using immunohistochemistry and immunoblotting. MAb TSd-1 reacted with elongating and matured spermatids in a stage-dependent manner. TSd-1 recognized a 94 kilodalton (kDa) peptide in the plasma membrane and cytosol. Additionally, an extremely weak ...

As a member of the large Ran-binding protein family, Ran-binding protein 9 (RANBP9) has been suggested to play a critical role in diverse cellular functions in somatic cell lineages in vitro, and this is further supported by the neonatal lethality phenotype in Ranbp9 global knockout mice. However, the exact molecular actions of RANBP9 remain largely unknown. By inactivation of Ranbp9 specifical...

BACKGROUND The Ayurvedic medicinal system claims Mucuna pruriens (MP) to possess pro-male fertility, aphrodisiac and adaptogenic properties. Some scientific evidence also supports its pro-male fertility properties; however, the mechanism of its action is not yet clear. The present study aimed at demonstrating spermatogenic restorative efficacy of MP and its major constituent L-DOPA (LD), and fi...

Rises of intracellular Ca2+ ([Ca2+]i) are key signals for cell division, differentiation, and maturation. Similarly, they are likely to be important for the unique processes of meiosis and spermatogenesis, carried out exclusively by male germ cells. In addition, elevations of [Ca2+]i and intracellular pH (pHi) in mature sperm trigger at least two events obligatory for fertilization: capacitatio...