the aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (bubalus bubalis) bulls. five healthy buffalo bulls (5 ejaculates from each bull) were used. each ejaculate was diluted at 37 ˚c with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 μg ml-1 sodium selenite and the sperm motility and...

Sperm viability (SV), the proportion of live sperm in a sample, is a widely applied measure of sperm quality but few studies test its robustness. At least three reasons make SV problematic as a surrogate for sperm quality. First, reviewing the ecological literature revealed that previously identified methodological pitfalls have not been overcome, including low cross-study standardization of pr...

The present study was conducted to test the post-thaw sperm characteristics (motility, viability, acrosomal and plasma membranes integrity) through addition of different concentrations of BSA (0.50, 1.00, 5.00, 10.00 and15.00 %) to semen diluents lacking egg yolk. Semen was collected from four mature buffalo bulls, twice weekly using artificial vagina. Sperm characteristics (motility, viability...

cyclophosphamide (cp) is one of the anti-neoplastic drugs. despite its numerous clinical applications, it has devastating effects on the testicles and declines the sperm quality in treated patients. this study was aimed to investigate the protective effect of crocin in improving the toxicity induced by cp in reproductive system. in this study, 24 male adult mice (6 to 8 weeks) were randomly div...

background: diabetes mellitus has a variety of structural and functional effects on the male reproductive system. diabetes results in reduced sperm parameters and libido. the present study aims to investigate the effects of royal jelly (rj) on reproductive parameters of testosterone and malondialdehyde (mda) production in diabetic rats. materials and methods: this experimental study was conduct...

four mature stallions were used to study the effect of enzymatic antioxidant catalase on freezability of turkmen stallion's sperm. collected semen from stallions were processed and pooled before being divided treatments (200×106  sperm/ml) and diluted with extenders supplemented with different levels of catalase [0 (control), 100 u/ml (cat100), 150u/ml (cat150) and 200u/ml (cat200)]. exten...

experiment was conducted to evaluate the effect of l-glutamine on coated sperm freezing by using four rams. semen was collected by artificial vagina contact with a tube containing tris-fructose-egg yolk 15%. samples were pooled and centrifuged. after removing supernatant and diluting pellet, samples were split into 5 equal part and added 0, 40, 80, 120 and 160 mm l-glutamine. samples were froze...

Egg yolk low-density lipoproteins (LDL) and soybean lecithin were evaluated as replacements for egg yolk in extenders used for the cryopreservation of brown-bear spermatozoa. The motility, viability and acrosomal status of post-thawed spermatozoa were analysed, and an egg-yolk extender was used as a control. The total antioxidant capacity of these extenders was tested. Soybean lecithin showed a...

BACKGROUND There is tremendous concern regarding the possible adverse effects of cell phone microwaves. Contradictory results, however, have been reported for the effects of these waves on the body. In the present study, the effect of cell phone microwaves on sperm parameters and total antioxidant capacity was investigated with regard to the duration of exposure and the frequency of these waves...