Allium cepa (onion) has a beneficial effect on disease treatment worldwide and has been used since ancient times as a medicinal and food source. Recently several reports have shown that onion has high antioxidant activity. As antioxidants have an essential effect on sperm health parameters, we investigated the effect of the fresh juice of onion bulbs on the spermatogenesis cycle in rats. Wistar...

A protocol is described for individually collecting two populations of sperm cells, Svn and Sua, from pollen of Plumbago zeylanica. Pollen grains were burst in 10 mM MOPS buffer containing 0.8 M mannitol (pH 4.6). Paired sperm cells released from pollen were separated using a microinjector. Svn and Sua were then collected individually with a microinjector, based upon known size differences. Col...

Assuring the highest possible quality of care to the patient is the first goal of the andrology laboratory. Quality control and quality assurance as they apply to the andrology laboratory are outlined, and special problems in quality control for sperm function assays are addressed. An example of the patient review process is presented. Quality of care also depends on the ability of the laborato...

OBJECTIVE Several methods for evaluating semen quality have been developed in addition to traditional semen analysis. Most of these methods are very complex and require expensive technical equipment as well as special knowledge to be performed. The aim of this study was to establish an easy and cost-effective sperm penetration test for bovine semen using artificial media. Additionally a standar...

Abbreviations: AI: Artificial Insemination; BCF: Beat Cross Frequency; PI: Propidiumiodide; ZBA: Zona Binding Assay; DFI: DNA Fragmentation Index; HZA: Hemizona Assay; SCSA: Sperm Chromatin Structure Assay; AOT: Acridine Orange Test; SCD: Sperm Chromatin Dispersion Test; ROS: Reactive Oxygen Species; VSL: Straight Line Velocity; LIN: linearity; VCL: Curvilinear Velocity; CTC: Fluorescent Chlort...

this study aimed at determining the physico-morphological attributes, in vitrofertilityand their interrelationships in semen of sahiwal bulls. semen samples werecollected weekly for 6 weeks from ten mature sahiwal bulls divided randomly into 5 groups. the mean (±sem) of ejaculate volume (ml), mass activity (score 0-5), progressive sperm motility (%), sperm concentration (106/ml), live sperm (%)...

The use of deep-frozen boar semen for artificial insemination (AI) is constrained by the need for high sperm numbers per dose, yielding few doses per ejaculate. With the advancement of new, intra-uterine insemination strategies, there is an opportunity for freezing small volumes containing high sperm numbers, provided the spermatozoa properly sustain cryopreservation. The present study aimed to...

Sperm cells are highly vulnerable to free radicals, and sperm quality and male fertility are critically affected by oxidative stress. Recently, sexual ornaments, particularly carotenoid-based colourful traits, have been proposed to depend on a male's capacity to resist oxidative stress, and thus to signal sperm quality. We conducted an experimental test of this hypothesis on great tits Parus ma...

BACKGROUND Within epidemiological and clinical research, missing data are a common issue and often over looked in publications. When the issue of missing observations is addressed it is usually assumed that the missing data are 'missing at random' (MAR). This assumption should be checked for plausibility, however it is untestable, thus inferences should be assessed for robustness to departures ...