It was reported that recombinase-A protein (RecA)-coated exogenous DNA was more likely to be integrated into mouse, goat and pig genomes. The objective of this study was to investigate whether integration of exogenous DNA into the rat genome is improved by the recombinase-mediated DNA transfer. Pronuclear microinjection of RecA-coated EGFP or OAMB DNA resulted in a production efficiency of tran...

The dimensions of spermatozoa from different levels of the reproductive tract were measured in 6 buffalo-bulls castrated under local anesthesia ; the same measurements were taken in the semen of 6 breeding buffalo-bulls. During transit from caput to cauda epididymis the sperm showed a significant increase in the length of head and main piece. However, a significant decrease was observed in midd...

In order to extend the static information of immunolabelling sulphogalactolipids in fixed boar spermatozoa, a fluorescent sulphogalactolipid analogue, galactose(3-sulphate)-beta 1-1'[(N-lissamine rhodaminyl)-12-aminodode-canoyl]-sphingosine, was incorporated into plasma membranes of living spermatozoa and its lateral distribution over the sperm head was studied. The fluorescent lipid was enrich...

sperms are highly specialized cells for delivering dna from male to the ovum. incredibly, wide degree of diversity in sperm morphology in their basic structures i.e. head, middle piece and tail is found across species. differences in terms of overall size of the sperm, shape and number of sperm produced are also incredible. one of the key for this variations or diversity in sperm may be associa...

H-Y (male) antigen was visually located on mouse sperm by electron microscopy, by use of the indirect hybrid antibody method and tobacco mosaic virus as the visual marker. Labeling was achieved by centrifugation of the sperm through a discontinuous gradient consisting of alternating layers of immune reagents and wash solutions. Treated sperm were examined topographically by preparation of plati...

Background: Sports, hypothermia and hyperthermia as some stressors inhibit male reproductive functions and are associated with subfertility or infertility. We investigated whether water temperature and chronic swimming are effective on mouse sperm parameters. Materials and Methods: Adult male mouse(N=35) were randomly divided into 7 groups: 1-controls 2-cold water (100C) with swimming 3- cold w...

The production of mature and motile sperm is a detailed process that utilizes many molecular players to ensure the faithful execution of spermatogenesis. In most species that have been examined, spermatogenesis begins with a single cell that undergoes dramatic transformation, culminating with the hypercompaction of DNA into the sperm head by replacing histones with protamines. Precise execution...

Intracytoplasmic sperm injection (ICSI)-mediated gene transfer has recently been shown to be an effective technique for producing transgenic pigs; however, the types of sperm pretreatment having the most beneficial effects on post-ICSI embryogenesis or transgenic efficiency have not been clarified. In the present study, we performed ICSI-mediated gene transfer using pig sperm subjected to vario...