Cryopreservation is an efficient way to store spermatozoa and plays a critical role in the livestock industry as well as in clinical practice. During cryopreservation, cryo-stress causes substantial damage to spermatozoa. In present study, the effects of cryo-stress at various cryopreservation steps, such as dilution / cooling, adding cryoprtectant, and freezing were studied in spermatozoa coll...

introduction: the purpose of this research was to study the effects of two cryoprotectants on viability, motility, morphology and fertilization rate of mouse sperm after freezing-thawing. material and methods: spermatozoa were isolated from caudae epididymis of nmri male mice (8 to 10 weeks-old) and divided to three groups of control, experimental 1 and 2. cryoprotectant solution for the first ...

In this study, we evaluated the effects of glutathione (L-gamma-glutamyl-L-cysteinylglycine; GSH) supplementation of the freezing extender on semen parameters during the cooling (2 hours at 5 degrees C) and freezing phases of the cryopreservation process to compensate for the decrease in GSH content observed during sperm freezing. To fully address these questions, we incorporated a new set of f...

The aim of this study was to evaluate the quality of boar sperm using two different freezing techniques. The protocols were proposed by Westendorf and Paquignon. In both methods the seminal plasma is removed. In the protocol Paquignon the seminal plasma is removed at environmental temperature and the Westendorf protocol suggests that the plasma withdrawal should be made at 15oC. The sperm suspe...

The objective of this study was to evaluate the importance of environmental and genetic factors affecting semen quality; sperm volume (SV), sperm concentration (SPCO), total sperm (TS), fresh sperm motility (FSM), total fresh motile sperm (TFMS), post thaw sperm motility (PTSM), total post thaw motile sperm (TPTMS), number of produced payout (NPP), number of fresh motile sperm in each milt ejac...

Computer-assisted sperm analyzers (CASA) have become the standard tool for evaluating sperm motility because they provide objective results for thousands of mammalian spermatozoa. Mammalian spermatozoa experience osmotic stress when the glycerol is added to the cells prior to freezing and removal from the cells after thawing. In order to minimize osmotic damage, cryoprotectants having lower mol...

Generally, spermatozoa from most boars survive poorly the customary process of cryopreservation. Post-thaw (PT) survivors often show unstable membranes, short life-span, and low fertilising capacity when artificial insemination (AI) is used. This thesis aimed at improving this situation by (i) freezing high sperm numbers in novel packages (MiniFlatPacksTM, MFP) and exploring their degree of PT-...

Chromosome stability was maintained in mouse spermatozoa after freeze-drying or freezing without cryoprotection in a simple Tris.HCl buffer containing EGTA (50 mM) and NaCl (50 mM). The ability of spermatozoa to activate oocytes spontaneously was not destroyed by freeze-drying or freezing without cryoprotection in this solution. Embryos derived after injecting oocytes with sperm heads from rehy...

Assisted reproductive techniques might prove themselves useful tools in producing buffaloes free of specific diseases (BFSD), which are in demand in South Africa. Freezing protocols for African buffalo semen must not only result in good post-thaw qualities, but must also be practical. Epididymal sperm from six mature African buffalo bulls was collected, diluted with three different semen extend...