Lectins are a diverse class of proteins distributed extensively in nature. Among these proteins; legume lectins display a variety of interesting features including antimicrobial; insecticidal and antitumor activities. Because lectins recognize and bind to specific glycoconjugates present on the surface of cells and intracellular structures; they can serve as potential target molecules for devel...

The aim of this study was to examine the distribution glycoconjugates in conjunctival epithelium partridge (Alectoris chukar) and quail (Coturnix coturnix). Eyelid samples harvested from both species were subjected routine tissue processing. Lectin histochemistry applied sections order demonstrate expression glycoconjugates. Six different HRP-conjugated lectins (Con A, UEA-I, PNA, HPA, MAA BSA ...

Cortical granule lectin (CGL) is released by the egg of the South African toad Xenopus laevis upon fertilization. The lectin binds to oligosaccharides in the extracellular matrix of the egg to form a physical block to prevent additional sperm penetration or polyspermy. To identify the oligosaccharides that bind to CGL, the lectin was immobilized on the surface of a matrix-assisted laser desorpt...

egg yolk is the most commonly used cryoprotective agent for sperm cryopreservation of several species.the objective of this experiment was to compare the effectiveness of egg yolk from four avian species(domestic chicken, duck, turkey and pigeon) on sperm quality following cryopreservation of the ram semen.ejaculates were collected, using an electroejaculator, from six fertile rams. samples wer...

BACKGROUND Despite interest in cryopreservation of individual or small number of human spermatozoa, to date, little data is available as regards its effectiveness. We systematically reviewed the outcome after cryopreservation of individual or small numbers of human spermatozoa in patients with severe male factor of infertility. METHODS We searched the MEDLINE, EMBASE, Cochrane Systematic Revi...

objective(s):althoughroutinely applied in assisted reproductive technology, human sperm cryopreservation is not a completely successful procedure. adverse effects of cryopreservation on the fertilization capacity, motility, morphology, and viability of spermatozoa have been proven; cryopreservation has also shown a role in sperm dna fragmentation and infertility. the post-thaw survival of sperm...

Although the development of semen cryopreservation in the African elephants (Loxodonta africana) has been accomplished, effective procedures for cryopreservation of Asian elephant (Elephas maximus) spermatozoa have not been established. In the present study, we investigate the freezing methods for conservation of Asian elephant spermatozoa under field conditions and identify the most suitable f...

Serial affinity chromatography by the use of immobilized lectin columns whose binding specificities have been precisely determined is a very sensitive and rapid method for the fractionation and structural assessment of oligosaccharides and glycopeptides. In this article, a possible scheme for separating a mixture of asparagine-linked glycopeptides is discussed with reference to the recent knowl...

Drosophila obscura group species produce two distinct sizes of nucleated sperm that differ only in head and tail lenghts. Between both sperm there is no differences in location of the acrosome and flagellum during spermiogenesis where each sperm type develops in its own bundle. Fertile sperm accumulate in the seminal vesicles. Fertilization is exclusively monospermic and in a previous study we ...

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