Two simple spectrophotometric methods have been developed for the determination of Cefuroxime Axetil in bulk drug and its tablet formulation. Method I is a simple UV spectrophotometric method. In this method the simple UV spectrum of Cefuroxime Axetil in 0.1 N HCl was obtained which exhibits absorption maxima (λ max) at 281 nm. Calibration curve was prepared by plotting the absorbance vs. conce...

Pterostilbene found in blueberries and Pterocarpus marsupium heartwood is structurally similar to resveratrol. It is used for the treatment of cancer, diabetes and fungal infections. Three spectrophotometric methods have been developed for the determination of Pterostilbene. Two first derivative methods and one difference spectrophotometric method were attempted. Two first derivative spectrosco...

A simple and sensitive UV spectrophotometric method was developed and validated for the determination of pregabalin in bulk, pharmaceutical formulations and in human urine samples. The method was linear in the range of 0.5-5.0 μg/ml. There is no generally accepted method for the determination of pregabalin. The absorbance was measured at 210 nm. The method was validated with respect to accuracy...

Titrimetric and spectrophotometric methods are described for the determination of oxcarbazepine (OXC) in bulk drug and in tablets. The methods use N-bromosuccinimide (NBS) and bromopyrogallol red (BPR) as reagents. In titrimetry (method A), an acidified solution of OXC is titrated directly with NBS using methyl orange as indicator. Spectrophotometry (method B) involves the addition of known exc...

Several researchers have proposed spectrophotometric modifications of the Winkler titrimetric method for measuring dissolved oxygen (DO). These modifications, although simple, are not widely used because of concern about accuracy, calibration, and possible sources of interference. Here we show, using natural samples from lakes and rivers as well as samples manipulated in the laboratory, that th...

The present study was aimed at reducing the time and labour used to perform DNA-DNA hybridizations for classification of bacteria at the species level. A micro-well-format DNA hybridization method was developed and validated. DNA extractions were performed by a small-scale method and DNA was sheared mechanically into fragments of between 400 and 700 bases. The hybridization conditions were cali...

A simple, sensitive, rapid and accurate spectrophotometric method was developed for the determination of risedronate, a bisphosphonate drug important for the treatment of a variety of bone diseases, in raw material and pharmaceutical formulations. The proposed method is based on complex formation between risedronate and Cu (II) ions in acetate buffer of pH5.5. The optimum conditions for this re...

A simple and sensitive extraction-spectrophotometric method for rapid and accurate determination of benzocaine in pure and dosage forms was developed. Benzocaine was effectively extracted as a 1:1 ion pair complex with dicyclohexyl-18-crown-6 and calmagite in acidic media into chloroform, followed by spectrophotometric determination at 486 nm. Molar absorptivity of the ternary complex at this w...

INTRODUCTION Because of its chemical diversity, the only way to standardise propolis is to specify multiple standards for different propolis types according to the corresponding chemical profile. So far, this has been done only for European propolis. OBJECTIVE To develop a rapid low-cost spectrophotometric procedure for quantification of bioactive prenylated flavanones in Taiwanese propolis. ...

Gabapentin is an anticonvulsant widely used in the treatment of epilepsy.An accurate and validated spectrophotometric method was developed as an alternative method to determine gabapentin in pharmaceutical products. This is a simple, accurate, precise, selective and visible spectrophotometric method. The method is based on the measurement of drug absorbance as a result of yellow solution format...