Due to the binding of the –COO- group with the mercury (II), gold nanoparticlesfunctionalized with glutathione can self-assemble to form a supermolecular network in the HAc-NaAc buffer solution. The aggregation of functionalized gold nanoparticles and mercury (II)caused the shift of maximum absorption peak of the UV-Vis spectrum from 520 nm to 609 nm.Based on this principle, a simple spectropho...

A very sensitive, optimized bioluminescent assay for certain kinase and creatine kinase MB activities is tested. We evaluated reagent blanks, sensitivity, precision and compared the results with those of the spectrophotometric immunoinhibition test. The main advantage of the new method is a detection limit of less than 1 U/l which, together with a high precision (s = 0.1 at detection limit), al...

An analytical procedure for examination of fountain pen and ballpoint pen inks was developed, using the PrinCe 550 capillary electrophoresis system with spectrophotometric detection. Samples were taken from ink lines in the form of paper microdots of 0.8 mm diameter. The ink sample was extracted from paper with pyridine-water solution (1:1). The detection was performed at wavelengths of 215, 37...

The levels of aspartate aminotransferase (AST/GOT) and alanine aminotransferase (ALT/GPT) in serum can help people diagnose body tissues especially the heart and the liver are injured or not. This article provides a comprehensive review of research activities that concentrate on AST/GOT and ALT/GPT detection techniques due to their clinical importance. The detection techniques include colorimet...

A flowthrough spectrophotometric sensor for the determination of cobalt at the nanogram per millilitre level using pyridoxal 4-phenylthiosemicarbazone as reagent and integrated preconcentration and detection in the flow cell is proposed. The method is highly selective for cobalt(II); it features detection and determination limits of 0.02 and 0.06 mug ml(-1) respectively, and a linear range of a...

Recently, detection of antioxidant activity of plant extracts has caught the attention of researchers. Determination of antioxidant activity is usually performed using stable free radicals. A number of assays is available for screening the antioxidant activity of food constituents, but few of these methods are applicable to lipophilic substances. EPR is the analytical technique that directly me...

due to the binding of the –coo- group with the mercury (ii), gold nanoparticlesfunctionalized with glutathione can self-assemble to form a supermolecular network in the hac-naac buffer solution. the aggregation of functionalized gold nanoparticles and mercury (ii)caused the shift of maximum absorption peak of the uv-vis spectrum from 520 nm to 609 nm.based on this principle, a simple spectropho...

The research includes, spectrophotometric determination for Metronidazole drug (MND) in pharmaceutical form (tablet), using multi-wavelengths technique, the results obeyed Beer’s law concentration ranges of between (0.5-25 µg.ml-1), recovery percentage Rec% (99.99- 100.05), RSD% (0.0011-0.0019), and detection limit (0.2042-2.9772 µg.ml-1) This method been successfully applied to MND tablet. als...

Two procedures are described for the detection of inhibitors of angiotensin converting enzyme (ACE). The first is a new agar-plate method useful as a screening tool. Two ACE inhibitors produced by culture A58365 were discovered using this plate test. The second method is a modification of a previously reported spectrophotometric procedure. Both procedures utilize p-nitrobenzyl-oxycarbonylglycyl...

Objective: To specify ritonavir and its commercial dosage form, the current study set out to design validate concise, precise, efficient high-performance liquid chromatography spectrophotometric methods. The developed spectroscopy chromatographic methods are reliable, accurate, specific for estimating ritonavir. Methods: method is superior previously described due shorter retention duration, us...