A Simple, accurate and reproducible spectrophotometric method by Hydrotrophic Solubilisation was developed for the estimation of Esomeprazole. In the present investigation, hydrotropic solution of urea (10M) was employed as a solubilising agent to solubilise the poorly water soluble drug, esomeprazole in the tablet form and determined with the help of spectrophotometric determination in ultravi...

Cartilage glycosaminoglycans (GAGs) were measured by a spectrophotometric assay in synovial fluid obtained from 30 normal bovine hock joints and 15 osteoarthritic human knee joints. Results were compared with those obtained by radioimmunoassay (RIA). The spectrophotometric method (dimethylmethylene blue (DMB) assay) was found to be simple, safe, and sufficiently reproducible to be of potential ...

A simple and derivative spectrophotometric method has been developed for the determination of mercury (II) using Diacetylmonoxime benzoylhydrazone (DMBH). Mercury (II) forms an orange coloured water soluble complex with the reagent in basic medium (pH 10.5).The molar absorptivity and Sandell’s sensitivity of coloured species are 2.01 x 10 L.mol cm and 0.0099 μg/cm respectively. Beer’s law is ob...

A rapid and sensitive direct spectrophotometric method for the determination of cerebrospinal fluid protein which usesas little as 0.1 ml. of sample has been described. The result is not affected by the ratio of albumin to globulin in the sample. Interfering substances are removed prior to spectrophotometric measurement with an automatic separation chamber employing the principle of gel filtrat...

A new simple rapid and sensitive spectrophotometric method has been developed for the determination of ketoprofen in pharmaceutical preparations. The method is based on the reaction of ketoprofen with an analytical reagent--Astra Phloxin FF--at pH 8.0-10.8 and followed by the extraction of formed ion associate in toluene with spectrophotometric detection (it has an absorption maximum at 563 nm,...

During spectrophotometric titrations of uracil in different alkalior alkali earth chlorides, specific changes in the U.V. absorption of uracil at PH 12.0 have been observed. The spectra of 4-methyl and 5-methyl uracil change in the same way whereas 1-methyl and 3-methyl uracil are not influenced even by strong divalent cations. From spectrophotometric and Potentiometrie titrations I I it could ...