Bisulfite sequencing is a widely used method for measuring DNA methylation in eukaryotic genomes. The assay provides single-base pair resolution and, given sufficient sequencing depth, its quantitative accuracy is excellent. High-throughput sequencing of bisulfite-converted DNA can be applied either genome wide or targeted to a defined set of genomic loci (e.g. using locus-specific PCR primers ...

Chromosomal interactions regulate genome functions, such as transcription, via dynamic chromosomal organization in the nucleus. In this study, we attempted to identify genomic regions that physically bind to the promoter region of the Pax5 gene, which encodes a master regulator for B cell lineage commitment, in a chicken B cell line, DT40, with the goal of obtaining mechanistic insight into tra...

Abstract TRP channel-associated factor 1/2 (TCAF1/TCAF2) proteins antagonistically regulate the cold-sensor protein TRPM8 in multiple human tissues. Understanding their significance has been complicated given locus spans a gap-ridden region with complex segmental duplications GRCh38. Using long-read sequencing, we sequence-resolve locus, annotate full-length TCAF models primate genomes, and sho...

386 students (181 female and 205 male) were selected randomly and asked to complete a demographic data sheet. rutter's internal and external locus of control and the beck depression inventory. data were analyzed by t-test and analysis of variance (anova). the results revealed, that 28% of students exhibited external locus of control, whereas, 72% exhibited internal locus of control. the me...