it has been suggested that two distinct species exist within what was originally known as entamoeba histolytica and e. dispar. these are entamoeba dispar for the nonpathogenic and e. histolytica for the pathogenic fonn. differentiation of these two organisms is of great clinical importance since they are morphologically indistinguishable and both fonns can infect the human intestinal cavity. a ...

Necrophagous fly is the most common insect evidence collected during a death investigation. We analyzed the DNA polymorphism among the five forensically important fly species, namely, Lucilia sericata, Aldrichina grahami, Chrysomya megacephala, Parasarcophaga crassipalpis and Musca domestica using inter simple sequence repeat (ISSR) method. Nine ISSR primers selected from 18 primers could ampli...

Battling malaria will be a persistent struggle without the proper means to diagnose the parasitic infection. However, the inherent limitations of microscopy, the conventional method of diagnosing malaria, affect the accuracy of diagnosis. The present study aimed to compare the accuracy of two different set of primers targeting the small subunit ribosomal RNA (ssRNA) and the dihydrofolate reduct...

background: potyviruses are accounted for 40% of viral diseases of various crops including vegetables and legumes. potyviruses can be transmitted through plant sap, seeds and many aphid species. due to ease of spread of these viruses detection of such viruses are crucial as to the control of the incited diseases. objectives: this study compared the efficiencies of two couples of primers in thei...

background:  aeromonas spp. can cause diarrhea and various infections in humans. access to rapid techniques with a high sensitivity and specificity is strongly needed for the identification of aeromonas species. the aim of this study was to evaluate two different methods including api 20e bacterial identification tests and the molecular detection using pcr primers specific for 16s-rrna and 23s-...

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Species-specific primers for four species of Neofabraea associated with apple and pear fruit diseases were used in multiplex PCR assays to identify 29 putative Neofabraea isolates, primarily isolated from bull’s-eye rot on pears from packing houses in Washington and Oregon. Apples were inoculated with these isolates, and tentative identifications based on morphology of conidia forming on wound-...

The morphological characters of Cirrochroa aoris (Large Yeoman) and C. tyche (Common Yeoman) are very similar making identification confusing and difficult. The genomic DNA of the two species was subjected to RAPD-PCR analysis with six decamer oligonucleotides, i.e. MA5, MA6, MA8, OPB12, OPT4 and OPT5. All of them produced discrete bands of various lengths revealing genetic variations as well a...

Three Hoplolaimus stephanus populations were characterized morphologically, both by morphometrics and by SEM observations. These populations were used to develop a rapid and accurate molecular identification method for the species, which is useful because of the high level of morphological similarity between H. stephanus and H. galeatus. Species-specific primers for H. stephanus, amplifying a d...