Background: Degradation of phytic acid to inorganic phosphate in domestic animals’ diets requires thermostable phytase. Although Basillus subtilis phytase shows a potential to be degraded phytate complex in high temperature, the enzyme activities and yields need to be increased to make them possible for industrial application. Methods: The phytase gene from Bacillus subtilis DR8886 was...

To explore the function of light-harvesting complex protein (LHCP) in Arabidopsis growth and development, the Leclere and Bartel seed collection was screened. In this collection randomly cloned cDNAs are expressed under the CaMV35S promoter. A pale green line has been identified and characterized in more details. Analysis of the inserted cDNA in the pale green line showed it encodes LHCB1 prote...

quality of protein fractions, according to cornell net carbohydrate and protein system (cncps), and digestibility coefficients, using tilley and terry procedure, of the fresh and ensiled amaranth (amaranthus hypochondriacus) forage were assessed. the concentration of crude protein (cp) and true protein (tp) in the fresh forage, were respectively 116 and 70 g/kg of dm. soluble protein (sp) and a...

multiple sclerosis (ms) is an autoimmune disease of central nervous system. since different types of immune cells are involved in ms pathogenesis, in this study we aimed to evaluate serum levels of several immunological components including soluble cd4 (scd4), scd8, scd163, and immunoglobulins as markers of activity of t-cells, macrophages, and b-cells in different types of ms. serum levels of ...

Nuclear cataract resulting from an overdose of selenite was characterized by a five-fold increase in nuclear urea-soluble protein. The origin of this urea-soluble protein was examined by two-dimensional electrophoresis, immunoblotting with monospecific antisera against rat lens crystallins, and tryptic mapping. Cataractous urea-soluble protein was primarily composed of insolubilized beta- and g...

background: withania somnifera l. dunal, (ashwagandha) belongs to the family solanaceae and is known as ‘indian ginseng’ in the traditional system of medicine. objectives: in the present investigation, comparative analysis was carried out between two different ecotypes of w. somnifera related to total soluble protein and antioxidant enzyme activity [catalase (cat), superoxide dismutase (sod) an...

The ability of pea protein isolates (PPI) to form complex coacervates with tragacanth gum was investigated. coacervate formation structurally compared three other PPI-polysaccharide interaction models: arabic and sodium alginate (known PPI) tara gum, a galactomannan. effects the pH protein/polysaccharide ratio were mainly investigated using turbidity zeta potential measurements. Regarding solub...

• S. obliquus was effectively fractionated by mild (un)catalyzed hydrothermal processes. Soluble sugars were mainly present as soluble oligosaccharides. Protein retained in the solid fraction. Dilute acid hydrolysis allowed higher oligosaccharides yield at conditions. Co-production of added-value marine biorefineries. Scenedesmus , a biotechnologically recognized microalga, grown 70 L vertical ...

Cataract is clouding of the eye lens that reduces the amount of incoming light and results in deteriorating vision. Blindness is thought to reach 75 million by 2020. Of these, unoperated cataract may be expected to account for at least 35 million. Thus, the burden of cataract is increasing remorselessly. Embelica officinalis is reported to have a very good antioxidant property and thus we hypot...

Background: Protein purification is the most complicated issue in the downstream processes of recombinant protein production; therefore, improved selective purification methods are important. Affinity-based protein purification method using His-tag and Ni-NTA resins is one of the most common strategies. MNPs can be used as a beneficial alternative for Ni-NTA resins. However, there is no data on...