BACKGROUND This study was performed to compare the prevalence of HPV infection and high risk HPV genotypes [16, 18] between monogamous and polygamous women, in Zabol, Iran. METHODS This cross sectional study was conducted in Zabol in 2006 - 2007. Two hundred sixty five married women attending the Gynecology Clinic for Cervical Disease Screening entered to this study. One hundred sixty two cas...

Human papillomavirus (HPV) is the main risk factor associated with the development of cervical cancer (CC); however, there are other factors, such as immunosuppression caused by the human immunodeficiency virus (HIV), that favor progression of the illness. This study was thus aimed at evaluating the functionality of classical PCR-based molecular tests for the generic identification of HPV DNA (...

This study developed a new multiplex polymerase chain reaction (m-PCR) for rapidly detecting clinically essential strains of V. parahaemolyticus. enables the detection total and potentially virulent strains. The m-PCR was by targeting species-specific transcriptional regulator toxR gene, sequences an outer membrane protein hypothetical encoded omp htp, respectively. htp were discovered original...

Objective : The study aimed to evaluate molecular and immunological methods propose a workflow using them for tuberculosis (TB) diagnosis routine. Methods A cross-sectional retrospective was performed, including 121 liquid cultures from TB laboratory located in the extreme south of Brazil. All were positive Mycobacterium complex (MTBC) by in-house Polymerase Chain Reaction (PCR) DNA extracted C...

In this study, two PCR-based methods (MSP-PCR and PCR-MP) were compared for their abilities to identify intraspecies variations of 23 isolates of Trichophyton rubrum, 78 isolates of Trichophyton interdigitale and 22 isolates of Microsporum canis, obtained mainly from patients in Lódź city. The results allowed to distinguish four types (containing two subtypes) characteristic for T. interdigital...