We have investigated changes in membrane protein organization of ATPdepleted red cells by employing intermolecular crosslinking of the neighboring membrane proteins with glutaraldehyde or catalytic oxidation. After oxidative crosslinking, the complexes of crosslinked proteins were separated by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS PAGE) and treated with a reducing agent,...

The fibrinolytic system of a patient with congenital deficiency of a2-proteinase (plasmin) inhibitor (a2Pl) was studied. The patient’s whole blood clot formed in vitro was lysed rapidly in several hours on incubation. This accelerated in vitro fibrinolysis was suppressed by an addition of purified a2Pl. The degree of suppression was proportional to the amount of a2PI added. In spite of the acce...

BACKGROUND Diagnosis of familial amyloidosis (FA) in Abyssinian cats usually is made on postmortem examination. HYPOTHESIS/OBJECTIVES Sequential analysis of serum SAA (sSAA), urinary SAA (uSAA), urinary protein:creatinine (UPC) ratio, or sodium-dodecylsulfate agarose gel electrophoresis (SDS-AGE) may facilitate early identification of cats with FA. ANIMALS Twenty-three Abyssinian cats belon...

We have investigated changes in membrane protein organization of ATPdepleted red cells by employing intermolecular crosslinking of the neighboring membrane proteins with glutaraldehyde or catalytic oxidation. After oxidative crosslinking, the complexes of crosslinked proteins were separated by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS PAGE) and treated with a reducing agent,...

An original synthetic route, based on the combination of a single-source precursor, UV-photodegradation and inverse w/o miniemulsion, is used to prepare Au nanoparticles (NPs) dispersed on titania. The source of the nanocomposite materials is the photolabile single-source precursor AuCl4(NH4)7[Ti2(O2)2(cit)(Hcit)]2·12H2O, which is suspended in a w/o miniemulsion consisting of different surfacta...

Bacterial profile modification is an important enhanced oil recovery technique used to direct injected water into a reservoir's low permeability zone containing trapped crude oil. During water flooding, the use of bacteria to plug the high permeability water zone and divert flow into the oil-bearing low-permeability zone will have a significant economic impact. However, during the field impleme...

A number of case reports on occupational asthma caused by herbal medicines have been issued, for example, on Sanyak, Chunkung, Banha, and Brazilian ginseng. Recently, cases of occupational asthma induced by Sanyak and Korean ginseng have been reported, but the pathogenic mechanisms involved are unknown. This study was carried out to evaluate the immunologic mechanism underlying Korean ginseng-i...

Fluorescent probes enable detection of otherwise nonfluorescent species via highly sensitive laser-induced fluorescence. Organic amines are predominantly nonfluorescent and are of analytical interest in agricultural and food science, biomedical applications, and biowarfare detection. Alexa Fluor 488 N-hydroxysuccinimidyl ester (AF488 NHS-ester) is an amine-specific fluorescent probe. Here, we d...