The extracellular human pathogen Trichomonas vaginalis is covered by a dense glycocalyx thought to play a role in host-parasite interactions. The main component of the glycocalyx is lipophosphoglycan (LPG), a polysaccharide anchored in the plasma membrane by inositol phosphoceramide. To study the role of LPG in trichomonads, we produced T. vaginalis LPG mutants by chemical mutagenesis and lecti...

A two-step purification of pneumococcal eclipse complex is described, which uses sucrose gradient sedimentation followed by agarose gel permeation chromatography. Purified complex contains, in addition to donor DNA single strands, macromolecular material that can be labeled with methionine or leucine during development of competence. This material co-chromatographed with eclipse complex DNA on ...

Two agro-based anionic surfactants containing an isosorbide moiety have been synthesized and their amphiphilic properties evaluated. Since isosorbide is now considered as an important platform chemical of the starch industry, these compounds could represent bio-sourced alternatives to the alkyl ether sulfates (notably lauryl ether sulfate, LES) that are based on petroleum-derived ethylene oxide...

Surfactant adsorption to fluid interfaces is ubiquitous in biological systems, industrial applications, and scientific fields. Herein, we unravel the impact of hydrophobic phase (air oil) role oil polarity on surfactants interfaces. We investigated anionic (sodium dodecyl sulfate), cationic (dodecyltrimethylammonium bromide), non-ionic (polyoxyethylene-(23)-monododecyl ether) at different inter...

In this study, moisture content, free fatty acids, peroxide, iodine value, unsaponifiable matter, saponification value, fatty acid composition and tocopherol contents of wheat germ oil obtained by SC-CO2 extraction and cold press technology were investigated. Moisture, free fatty acid (FFA), peroxide value, iodine value, unsaponifiable matter and saponification value of cold pres...

The dissociation of the extracellular hemoglobin of the earthworm Lumbricus terrestris was investigated. At neutral pH, its sz00,~ = 58.9 f 0.2 S and D:o,w = 1.61 f 0.05 X 1O-7 cm2 s-l corresponded to a molecular weight of 3.23 f 0.18 x 106. A minimum molecular weight of 24,000 per heme group was determined from the iron and heme contents, 0.221 f 0.011 weight % and 2.78 =k 0.14 weight %, respe...

The Ascaris suum intestinal basement membrane was solubilized on reduction with P-mercaptoethanol in 1% sodium dodecyl sulfate and 1% sodium dodecyl sulfate, 8 M urea to the extent of 90% and lOO%, respectively. The reduced membrane consists of at least 17 polypeptides ranging in molecular weight from 22,500 to greater than 400,000, as determined by sodium dodecyl sulfate-polyacrylamide gel ele...