The effects of crude plasma and ethanol-extracted swamp eel at different concentrations (0, 0.25, 0.5, 0.75, 1, 1.5, 2 mg/g) as inhibitor protease in the quality tilapia (Oreochromis mossambicus) surimi were investigated. parameters analyzed gel strength, expressible moisture, whiteness, microstructure, protein solubility, hydrolysis pattern by sodium dodecyl sulfate-polyacrylamide electrophore...

Our objectives were to determine if milk casein as a percentage of true protein (CN%TP) estimated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) is equivalent CN%TP Kjeldahl, and the proportion (CN), proteolysis products (CNPP), serum (SP) from (TP) that goes into Kjeldahl noncasein nitrogen (NCN) filtrate stays in NCN precipitate using SDS-PAGE. Raw samples collected 1...

Detergent (Lubrol WX)-solubilized sodium-potassium-activated adenosine triphosphatase ((Na+ + K+)-ATPase) of electrophorus electric organ contains two major constituent polypeptides with molecular weights of 96,000 and 58,000 which can be readily demonstrated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. These two polypeptides can be clearly separated and can be obtained in mill...

We have purified the membrane-intrinsic glycerol3-phosphate dehydrogenase from both normal and hyperthyroid rat liver mitochondria by extraction with Triton X-100, hydrophobic affinity chromatography, ion exchange chromatography, gel filtration, and FAD-linked Sepharose 4B affinity chromatography. The yields in both cases were over 20‘70, and purification ranged from 800to 650-fold in mitocho...

Staphylococcus aureus 462 is one of three bacteriocin-producing strains selected for study from 200 isolates of staphylococci of animal origin. These bacteriocins are specific in their activity, inhibiting the growth of certain strains of S. aureus and other gram-positive species, but not gram-negative organisms. Staphylococcin 462 was not found in significant concentrations in the supernatant ...

Poly(A)+ RNA was isolated from liver-free polysomes of rats maintained on a 60% casein diet by sodium dodecyl sulfate-phenol-chloroform extraction and oligo(dT)-cellulose chromatography. Poly(A)+ RNA translated in a rabbit reticulocyte lysate system produced a polypeptide of 49,000 daltons that was immunoprecipitated by monospecific, affinity-purified IgG antibodies to ornithine aminotransferas...

Three alkaline RNases were purified from human pancreatic juice to near homogeneity as judged by sodium dodecyl sulfate and native polyacrylamide gel electrophoreses. The molecular weights of these RNases determined by sodium dodecyl sulfate electrophoresis were 27,000, 19,000, and 13,000. The activities of these three RNases were increased by addition of Mg2+, Ca2+, Co2+, K+, Na+, spermine, an...

The purification of a lipase from skim milk is described. The enzyme had the characteristics of a lipoprotein lipase, i.e. its activity against emulsified long chain triglyceride was stimulated more than ZO-fold by addition of suitable amounts of serum to the assay system and the activity was almost completely inhibited by 1 M NaCl. After an initial fractionation of the skim milk, the main puri...

An actin-like protein has been identified in cell extracts from the prokaryote Mycoplasma pneumoniae. This protein bears a striking resemblance to actin from vertebrates: (i) the solubility of the protein during isolation is analogous to that of actin bound to myosin (soluble in high ionic strength salt solution and insoluble at low ionic strength), (ii) sodium dodecyl sulfate treatment of the ...